FastQCFastQC Report
Fri 30 Jun 2023
SRR1944923.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944923.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36551062
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length25-48
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA20043045.483572542981104No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA3718731.0174068266470615No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG3603940.9860014464148812No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC3091920.8459179653931806No Hit
AAGAGGTGCACAATCGACCGATCCTGA2870450.7853260187077463No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC2180700.5966174115542798No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1883700.5153612226096194No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1752920.4795811404877921No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1661390.4545394604403013No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1496750.40949562559905917No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1471120.4024835174419829No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT1380000.3775540092378164No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1209450.3308932583135341No Hit
CAATATAGATGGATACGAATAAGGCGTCC1129970.30914833610033No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT1061110.2903089382190865No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC1045300.28598348250455763No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT975410.2668622870656946No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT882320.24139380683384795No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA861820.235785214667634No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC707570.1935839784901462No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT706890.19339793738414496No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT693790.18981391019500335No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCT653040.17866512332801712No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT610920.16714151835041072No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT609940.16687340028587952No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT576970.1578531425434369No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT572490.15662745996272284No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG563650.15420892558470667No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA512120.1401108400078772No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC507150.13875109839489752No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG503110.13764579535336074No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGG455710.12467763590562704No Hit
CTTGCGTGCCTTGTTGTAGACGGCCTTGG452350.12375837397009148No Hit
ACTTGCGTGCCTTGTTGTAGACGGCCTTGG449810.12306345572120449No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGG448820.12279260175805562No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT444930.1217283371957838No Hit
ATGTACACGGAGTCGACCCGCAACGCGA410630.1123442049371917No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGT402890.11022661940711873No Hit
ATCTTTGAACGCACATTGCGCCCCTTGGT402880.11022388350850107No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA393950.10778072604292592No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGC387950.10613918687232672No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC374590.10248402631912583No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA373060.10206543383062304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)