FastQCFastQC Report
Fri 30 Jun 2023
SRR1944936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22825808
Total Bases680.6 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA17937157.858276035617227No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG13998856.1329044737430545No Hit
AAGAGGTGCACAATCGACCGATCCTGA6042132.6470607305555185No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC3235281.4173780836148275No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA3062741.341788207453598No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1462680.6408009740553324No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1288070.5643042296684525No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1258510.5513539761659259No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1013920.44419895234376805No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC983500.430871932332034No Hit
GTTTCTTTACTTATTCAATGAAGCGGAG982240.4303199255859858No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG974500.4269290270031186No Hit
CAATATAGATGGATACGAATAAGGCGTCC819110.3588525759964335No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC768730.33678106816634923No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG636350.2787853117839246No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT622450.2726957135537108No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT536140.234883251449412No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT520920.22821536043762394No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG507050.22213890522517318No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGC504990.22123641800544366No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA463190.20292381325559208No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG426780.18697257069716874No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT407280.17842960915118536No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT392820.17209467458939462No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC385920.1690717805038928No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG363000.15903051493292153No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT360340.157865167357931No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT358880.1572255404934625No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT354410.15526723084676786No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGC349540.15313368096323252No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGG334090.14636502681526106No Hit
ACAAGAGGTGCACAATCGACCGATCCTGA324850.14231697734424123No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT257450.11278899743658581No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA246620.10804436802412426No Hit
CTTGCGTGCCTTGTTGTAGACGGCCTTGG238940.10467975547678313No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGT237050.10385174535771088No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGT234930.10292297210245525No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA233530.10230963127351286No Hit
AAAGAGGTGCACAATCGACCGATCCTGA231450.10139838204194129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)