FastQCFastQC Report
Fri 30 Jun 2023
SRR1944938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29893433
Total Bases889.3 Mbp
Sequences flagged as poor quality0
Sequence length25-49
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAG318151710.64286259794919No Hit
CAAGAGGTGCACAATCGACCGATCCTGA21813117.296957161126325No Hit
AAGAGGTGCACAATCGACCGATCCTGA6834912.2864252493181363No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC5754851.9251218152160712No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA3355371.122443849122314No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG2440990.8165639590474604No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1915650.6408263647738284No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1757110.5877913052007108No Hit
GTTTCTTTACTTATTCAATGAAGCGGAG1509500.5049604038452191No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1343810.4495335146016853No Hit
CAATATAGATGGATACGAATAAGGCGTCC1161260.3884665906388203No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1084980.3629492805326173No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG1006900.3368298314884075No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT977390.32695809812141685No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGC901560.3015913227497156No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG767030.2565881275663454No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG741300.2479808859691692No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC690180.23088014012977365No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT615140.20577763684753106No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT558320.186770117704447No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT542430.18145456896837509No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG511250.17102418447556691No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA465040.15556593985040126No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT453470.1516955245655459No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGC442840.14813955961498299No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT442050.14787528752552442No Hit
TTTACCCAATCCTAATTCAGGGAGGTAGTG397540.13298572967514302No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT387490.12962378727127125No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC376330.12589052585562857No Hit
CAATATAGATGGATACGAATAAGGCGTC344360.11519586927336181No Hit
ACAAGAGGTGCACAATCGACCGATCCTGA337800.11300140736595894No Hit
AAAGAGGTGCACAATCGACCGATCCTGA316530.1058861322485109No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT301420.10083151038557533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)