FastQCFastQC Report
Fri 30 Jun 2023
SRR1944939.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944939.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36174506
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length25-48
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAG15163484.191758693263151No Hit
CAAGAGGTGCACAATCGACCGATCCTGA10472292.8949365611240134No Hit
TCCGATATAGTGTAACGGCTATCACATCACG3234720.8941988039864318No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC3200630.884775040189906No Hit
AAGAGGTGCACAATCGACCGATCCTGA2023590.5593967198888631No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1851560.5118411292195669No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1569940.4339907226376499No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG1384190.3826424056765281No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT1383730.38251524429939693No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1290780.35682035298560816No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT1247540.34486718353527757No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1227840.3394213593407468No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC1158200.3201702325941922No Hit
CAATATAGATGGATACGAATAAGGCGTCC1149360.3177265226510626No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1036990.2866632097201272No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT928570.2566918260058617No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG864550.23899427956251842No Hit
AACTATGCCGACTAGGGATCGGGTGGTG834000.2305491054943501No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC627280.17340388836270493No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC595840.1647126846735654No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT551880.1525604800242469No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA540390.14938420997373122No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT534840.14784998031486593No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT523050.14459077893143862No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT485840.13430452927263195No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT470630.13009991069401197No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT462560.12786905783868896No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA453380.12533135905159284No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA427400.11814950562144512No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG423740.11713774335992315No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT408130.11282254967075432No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA402690.11131872816728997No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT399700.11049217921593733No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG376160.10398483396013755No Hit
TTGCTCCACACAATATAGATGGATACGAAT373090.10313616998667516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)