FastQCFastQC Report
Fri 30 Jun 2023
SRR1944948.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944948.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26182323
Total Bases791.7 Mbp
Sequences flagged as poor quality0
Sequence length25-49
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA13631275.206287463492067No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG10870354.151789739970743No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA6968652.6615858340759146No Hit
AAGAGGTGCACAATCGACCGATCCTGA4297691.6414471702911924No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC3046851.1637049928686618No Hit
ATGTACACGGAGTCGACCCGCAACGCGA1955760.7469772640112949No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1881820.7187368362998195No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1803040.6886478331200788No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG1275050.48698887413465947No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1158470.44246264932259827No Hit
TCAATATAGATGGATACGAATAAGGCGTCC1064250.40647653762425895No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT992390.37903053903964135No Hit
AACTATGCCGACTAGGGATCGGGTGGTG817450.3122144662259342No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA799790.3054694573892469No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT697090.266244519250641No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA685980.2620011982893955No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG601500.22973515375240006No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA596830.2279515075877721No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA565120.2158402827739922No Hit
CAATATAGATGGATACGAATAAGGCGTCC499700.1908539589859922No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC477920.18253536937879805No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC477000.18218398726499554No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC447990.17110399256780998No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG438900.16763218450860912No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA406670.15532235241311476No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG386270.14753083597662436No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC368520.1407514528027173No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT364830.1393421049767051No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT342580.1308440049418075No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC335040.1279641993569478No Hit
GTTTCTTTACTTATTCAATGAAGCGGAG332880.12713921526367236No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC306190.11694531459259747No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT304250.11620435665697043No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA299690.1144627235711667No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGC296860.11338184163414376No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT290660.11101383173677906No Hit
CCGATATAGTGTAACGGCTATCACATCACG288450.1101697507894926No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC286940.10959302579836021No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT274280.10475770236277354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)