FastQCFastQC Report
Fri 30 Jun 2023
SRR1944949.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944949.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20765618
Total Bases638.5 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA6879063.3127162408554374No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA3752951.8072903007269034No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG3578741.7233968187221782No Hit
AAGAGGTGCACAATCGACCGATCCTGA1419020.6833507194440348No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1162570.5598533113726738No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1037850.4997924935342642No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT731120.35208198474998437No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT712590.3431585806885208No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT705650.33981651786139955No Hit
TCAATATAGATGGATACGAATAAGGCGTCC654960.3154059753964462No Hit
AACTATGCCGACTAGGGATCGGGTGGTG616180.2967308750454718No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC534340.25731957507838193No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG483880.23301979262066747No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT448120.21579901932126463No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA372240.1792578482374086No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC347570.1673776335479156No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA334470.1610691287877876No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA331500.15963887999865933No Hit
CAATATAGATGGATACGAATAAGGCGTCC308810.14871216450191851No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG306420.1475612235571318No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA305080.14691592612365306No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG302810.14582277300873012No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC288310.13884007690019146No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA284600.1370534698269033No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA275510.13267604171472286No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC272400.13117837379075353No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA257040.12378153156819122No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT253450.12205271232476683No Hit
ATGTACACGGAGTCGACCCGCAACGCGA242570.11681328241711852No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAA241540.11631727021078785No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT236460.113870918746555No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC234500.11292705085878012No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT219670.10578543821811613No Hit
AGGTTCTTTACTTATTCAATGAAGCGGAG210970.10159582055299295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)