FastQCFastQC Report
Fri 30 Jun 2023
SRR1944950.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944950.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20395543
Total Bases622.9 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA9985974.896153046771052No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA5925742.905409284763833No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG5822442.8547609641969327No Hit
AAGAGGTGCACAATCGACCGATCCTGA2134081.0463462531985541No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1981830.9716975909883842No Hit
TCCGATATAGTGTAACGGCTATCACATCACG854950.4191847208971098No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG708600.3474288475673337No Hit
AACTATGCCGACTAGGGATCGGGTGGTG629360.3085772219940406No Hit
TCAATATAGATGGATACGAATAAGGCGTCC584660.2866606689510547No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC584640.2866508628870533No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT582090.285400589726883No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA575530.2821842007344448No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT567400.2781980357179017No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT530410.2600617203474308No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA453820.22250939825431468No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC359380.1762051640400062No Hit
AGGTTCTTTACTTATTCAATGAAGCGGAG348130.1706892530392547No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC347550.17040487718321598No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG324680.1591916429976883No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA309800.1518959313806943No Hit
CAATATAGATGGATACGAATAAGGCGTCC258270.12663060748125216No Hit
CATGAGGTGCACAATCGACCGATCCTGA251020.12307590928076785No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA248920.12204627256062758No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC247670.1214333935605441No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT246830.12102153887248797No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC240100.11772179833603842No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA206680.101335865389806No Hit

[OK]Adapter Content

Adapter graph