FastQCFastQC Report
Fri 30 Jun 2023
SRR1944951.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944951.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23931990
Total Bases741.5 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA6861142.866932503314601No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA6633302.7717293881536804No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG4848812.026078901085952No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT1983280.828715037905331No Hit
AAGAGGTGCACAATCGACCGATCCTGA1939830.8105594227642583No Hit
ATGTACACGGAGTCGACCCGCAACGCGA1544560.6453955563244009No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1474760.6162295738883394No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1223280.5111484669682713No Hit
TCAATATAGATGGATACGAATAAGGCGTCC1157090.4834909257441609No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1083140.45259086269048254No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT773220.32309055786835944No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG740750.3095229439758248No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA615740.2572874215641909No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC587820.24562102858976625No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA560820.23433905830647594No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA518010.21645086764619242No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT481870.20134974149663273No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC428810.1791785806362112No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT403630.16865709872016493No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA402250.16808046468346344No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA381380.1593599195052313No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC360390.1505892322368512No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC355010.1483411951952178No Hit
AACTATGCCGACTAGGGATCGGGTGGTG336620.14065691988004342No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT331270.138421418360947No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA327090.13667480222079317No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT326120.13626948699209718No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT307710.12857685466189814No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC291940.12198734831495417No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC287780.12024908918982499No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG276970.11573212256899656No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG262240.10957718100333487No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGT247030.10322167107708134No Hit
CAATATAGATGGATACGAATAAGGCGTCC246220.10288321196858263No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA245950.10277039226574973No Hit
TCTACTTGCGTGCCTTGTTGTAGACGGCCTTGG243030.10155026807214945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)