FastQCFastQC Report
Fri 30 Jun 2023
SRR1944952.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944952.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21656332
Total Bases669.7 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAGGTGCACAATCGACCGATCCTGA4583582.1165080032943715No Hit
CAAGAGGTGCACAATCGACCGATCCTGA4193931.9365837206411503No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG3843491.7747649971380195No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1443010.6663224409378282No Hit
CAATAGGTGCACAATCGACCGATCCTGA1356160.6262186966841845No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1033040.47701522122952306No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT929320.42912160748182104No Hit
AAGAGGTGCACAATCGACCGATCCTGA885730.40899354516729797No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT764580.3530514770460667No Hit
TCAATATAGATGGATACGAATAAGGCGTCC692150.3196062934387966No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT613950.2834967620555503No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG596690.27552680666328905No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT531490.24542013855347247No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA402690.18594561627518455No Hit
AACTATGCCGACTAGGGATCGGGTGGTG391570.18081085938283548No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA347730.1605673573899772No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA338180.15615756167757308No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC338010.15607906269630517No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC333530.15401038366053865No Hit
ATGTACACGGAGTCGACCCGCAACGCGA332820.1536825349740667No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG303350.14007450569191496No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC287760.13287568735093275No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA259090.1196370650394536No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC253760.11717589109734741No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG249970.115425825573786No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA247360.11422063533196665No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT240610.11110376401691661No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT226150.10442673302200946No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT224820.10381259393326626No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC218350.10082501505795163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)