FastQCFastQC Report
Fri 30 Jun 2023
SRR1944953.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944953.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27994333
Total Bases867 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAG8613763.0769656130046035No Hit
CAAGAGGTGCACAATCGACCGATCCTGA6155702.198909329256032No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA5637902.0139433220287835No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC2297760.8207946944119011No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT2043530.7299798855718406No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1894410.6767119616673846No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG1403870.5014836395637646No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1052290.3758939353904235No Hit
TCAATATAGATGGATACGAATAAGGCGTCC1039800.37143231810523936No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT909800.324994347963211No Hit
AAGAGGTGCACAATCGACCGATCCTGA784900.28037817511136987No Hit
AACTATGCCGACTAGGGATCGGGTGGTG741540.2648893259932287No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT694930.24823952762153684No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC641350.22909993961992237No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA597620.2134789208944539No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC549990.19646476306472457No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA535910.19143517368318794No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC467890.16713739884425893No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA443390.1583856275482613No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA442740.15815343769755114No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG431800.15424550390252198No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA421930.1507197903232772No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA420190.15009823595368393No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT411810.14710477295529778No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC391190.13973899646046217No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC384970.13751711819674362No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT368710.13170879977744068No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT364900.1303478100371243No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC331170.11829894286104262No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG329140.1175737960965171No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT323340.11550194819787277No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG318350.11371944457472874No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT316210.11295500414315997No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAA307180.10972935129406368No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT290300.10369955947869879No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC288470.103045855745161No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG283300.10119905339412802No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA281430.1005310610543927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)