FastQCFastQC Report
Fri 30 Jun 2023
SRR1944960.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944960.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22587414
Total Bases691.9 Mbp
Sequences flagged as poor quality0
Sequence length25-49
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA7201883.188448221651226No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG6752342.989425881156648No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA4876522.158954539904391No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1703040.7539774141475425No Hit
ATGTACACGGAGTCGACCCGCAACGCGA1248560.5527680149662109No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG1078230.4773587627162631No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1010290.44728006490694333No Hit
AAGAGGTGCACAATCGACCGATCCTGA837060.37058691180849657No Hit
TCAATATAGATGGATACGAATAAGGCGTCC692810.30672391270643024No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT644680.2854155858656507No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT631650.27964688653601516No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT600300.2657674756393096No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT489120.21654537345443797No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC457310.20246230931969458No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA455390.2016122784131021No Hit
AACTATGCCGACTAGGGATCGGGTGGTG450510.19945178319217952No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA367120.1625329929313732No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC362670.1605628692155729No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC352280.15596296238250204No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG335300.14844550155232467No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC317740.14067126055244747No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC317710.140657978819532No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT317210.1404366166042735No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA303490.1343624374175813No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG303400.13432259221883477No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT299270.1324941403207999No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT289270.12806689601563065No Hit
CAATATAGATGGATACGAATAAGGCGTCC265580.11757875425668471No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA258960.11464791852666269No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT248390.10996832129609879No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT235790.10438999347158555No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA230580.10208339918859237No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC228930.10135290387823943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)