FastQCFastQC Report
Fri 30 Jun 2023
SRR1944961.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944961.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27431659
Total Bases844.4 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA10186723.713490314238741No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG8101582.9533685877328817No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA5519882.012229737909763No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT2402020.8756378897827507No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC2093970.7633406349940409No Hit
AAGAGGTGCACAATCGACCGATCCTGA1999960.7290700135926886No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1739890.6342634982448564No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1385320.5050077357698272No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG1072810.39108462233363284No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT961250.3504162836086581No Hit
AACTATGCCGACTAGGGATCGGGTGGTG937020.3415834237367853No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT885680.32286782217582977No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT777060.28327123780592345No Hit
TCAATATAGATGGATACGAATAAGGCGTCC702350.2560362827490674No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA695530.2535501042791469No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA675370.24620093155867823No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC586520.21381134841316013No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG566630.20656060211305485No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC549570.20034151051527724No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA548000.19976917910797887No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC532130.1939838928443956No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA516200.1881767340429538No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA453540.16533451367268748No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT432300.15759163527076508No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT429960.1567386062942821No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA402850.14685586460519942No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG364620.1329194125663344No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC349830.1275278319842048No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT344010.12540619581192666No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT329360.120065651151467No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC327970.11955893735774421No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT324950.11845801961886447No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA320110.11669363489827575No Hit
CAATATAGATGGATACGAATAAGGCGTCC305470.11135673566079253No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG298520.10882316669217854No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT294170.10723740769743456No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT283960.1035154308385067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)