FastQCFastQC Report
Fri 30 Jun 2023
SRR1944962.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944962.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19505928
Total Bases593.8 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA9684994.965152132213346No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA4579232.3476094036643627No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG3678151.8856575293418494No Hit
AAGAGGTGCACAATCGACCGATCCTGA2324521.1916992618859252No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1739620.8918417006358272No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1293590.6631778811036316No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1291220.6619628658528833No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1186900.6084816882334437No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1018320.5220566793848516No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT940890.48236105454711No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA882990.4526777705731304No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT805480.4129411325623677No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC538040.2758340951530222No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG528890.27114321348874043No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG508550.2607156142481404No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG508360.26061820796221535No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA455140.2333341946099668No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT420250.21544732452616458No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT394700.20234874239256906No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA386700.19824742509046478No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC352910.18092448613570195No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG349520.17918655292893526No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT348720.1787764211987248No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT321060.16459611662669932No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG317100.16256596456215772No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC305940.15684462692572226No Hit
CTAGAGGTGCACAATCGACCGATCCTGA293180.15030302582886598No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT286700.1469809588141615No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC278570.14281299510589807No Hit
CATGAGGTGCACAATCGACCGATCCTGA270590.13872193109704906No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG269980.1384092056527636No Hit
CCGATATAGTGTAACGGCTATCACATCACG266110.13642519340787065No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT257760.13214444347379933No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTC256360.13142671294593108No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT244310.12524910375963655No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGG230700.11827173769943168No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT217490.11149943750433201No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG213250.10932573933421674No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT209200.10724944745002649No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT208780.107034128291666No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA206350.10578835316115184No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT206170.10569607352185449No Hit
TCAATATAGATGGATACGAATAAGGCGTCC203570.1043631453986706No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGT203390.10427086575937325No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC201060.10307635709513538No Hit
CCGTGATAGTTTAATGGTCAGAATGGGC195730.10034385444260842No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTC195510.10023106821680057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)