FastQCFastQC Report
Fri 30 Jun 2023
SRR1944963.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944963.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20382645
Total Bases637 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAGGTGCACAATCGACCGATCCTGA6061992.974093892132253No Hit
CAAGAGGTGCACAATCGACCGATCCTGA2204641.081626059817065No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG2114431.0373678195347071No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1683950.826168537007832No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1647700.8083837990604261No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1172600.5752933439207718No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC1065120.5225622091735396No Hit
TCAATATAGATGGATACGAATAAGGCGTCC878140.43082730430716915No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT855160.41955300698216547No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG851530.41777208012012174No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC842830.41350374301274445No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG806660.3957582541421882No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA687940.3375126240976085No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA671440.32941750199741004No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC667950.3277052610198529No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG645450.3166664581559459No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT553360.271485864567626No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA540490.2651716693294712No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG513360.25186132614290246No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA509800.25011474222310204No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG495810.243251059909055No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC495700.24319709242838697No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC484240.2375746621697037No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG479650.23532274638546669No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT477970.23449851577162828No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT461520.2264279243444607No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG460750.22605015197978479No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT437920.2148494466738738No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC414830.2035211818682021No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT385500.18913148906827354No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA378360.18562850895946037No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG377200.18505939734514337No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA360050.17664537649554315No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT351480.17244081913804613No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT343450.16850119304928285No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT328430.16113217887079914No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA319750.15687365403263415No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC316800.15542634432381075No Hit
AAGAGGTGCACAATCGACCGATCCTGA315730.15490138792094943No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG303070.1486902215095244No Hit
AACTATGCCGACTAGGGATCGGGTGGTG302780.14854794360594517No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG301620.14797883199162817No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA298240.14632055849473902No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG271360.13313286867332477No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG266390.13069451977405286No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG262130.1286045064318198No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA259390.12726022554972624No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC255230.125219273553555No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT254210.12471884782372454No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA246770.12106868367672595No Hit
CCGATATAGTGTAACGGCTATCACATCACG239410.11745776860657682No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGGT226400.11107488748393547No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT225420.11059408629252976No Hit
TTTGTGGCGTCGCTGAACCATAGCAGGCTAGC224130.10996119492833242No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT222820.10931849129492271No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT216420.10617856514696694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)