FastQCFastQC Report
Fri 30 Jun 2023
SRR1944964.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944964.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14366950
Total Bases448.1 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAGGTGCACAATCGACCGATCCTGA3078692.142897413856107No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG2037731.418345577871434No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1303880.9075551874266982No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC896770.6241895461458417No Hit
TCCGATATAGTGTAACGGCTATCACATCACG808530.5627708038240544No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC797980.5554275611733875No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG754790.5253655090328846No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT680240.4734755811080292No Hit
TCAATATAGATGGATACGAATAAGGCGTCC529190.3683384434413706No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA506970.3528723911477384No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA454830.31658076348842307No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA386760.2692011874475793No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT383580.2669877740230181No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT382410.26617340493284936No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC343610.23916697698537268No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA336510.23422507908776743No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC327920.22824607867362243No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG327490.2279467806319365No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG324270.22570552552907888No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG319970.22271254511221938No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG312170.21728341784442765No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT301140.20960607505420428No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA275030.19143241954625026No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC274360.19096607143478608No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT269680.18770859507411108No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT252300.1756113858543393No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG248270.17280633676598026No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC247740.17243743452855337No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT246550.17160914459923646No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA240030.1670709510369285No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC238670.16612433397485202No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT238140.16575543173742513No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC234790.16342369118010433No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA227340.15823817859740585No Hit
ATGTACACGGAGTCGACCCGCAACGCGA219620.15286473468620687No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG215750.1501710523110333No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT208930.14542404616150262No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA203410.1415818945566039No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA184710.12856590995305198No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT176670.12296973261548207No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG172420.12001155429649299No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT172180.11984450422671479No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC172110.11979578128969615No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG159890.1112901485701558No Hit
AACTATGCCGACTAGGGATCGGGTGGTG156430.10888184339752001No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGGT148260.10319518060548691No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT147000.10231816773915131No Hit
TTTGTGGCGTCGCTGAACCATAGCAGGCTAGC143750.10005603137757144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)