FastQCFastQC Report
Fri 30 Jun 2023
SRR1944965.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944965.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28290539
Total Bases885.2 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAGGTGCACAATCGACCGATCCTGA6323942.2353550775402335No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG3820821.350564582739127No Hit
CAAGAGGTGCACAATCGACCGATCCTGA3706531.3101659180123786No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1902550.6725039773897556No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1586950.5609472481241874No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1537830.5435845531256933No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1497740.5294137379284289No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1493360.5278655171610551No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC1417260.500966065015587No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT1415270.5002626496441089No Hit
TCAATATAGATGGATACGAATAAGGCGTCC930890.3290463995754906No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG922670.3261408345737068No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC907990.32095182067757705No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT817210.2888633546359792No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA794790.2809384437673669No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG784230.27720574712273954No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT770020.2721828665053006No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA768220.2715466113954209No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT737410.26065604476464727No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG730620.2582559491001568No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA685700.24237784935804865No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT654610.23138830971018262No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG627860.22193285182724865No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA616780.21801634815087825No Hit
AACTATGCCGACTAGGGATCGGGTGGTG592460.2094198346662819No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG576720.20385613720544526No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG571860.20213824840877018No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC563700.199253891910649No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC556760.19680077498700185No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT549340.19417799003405345No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC536180.18952625823071098No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT528900.18695295978630877No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC506710.17910934818173666No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC491810.17384256977217719No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG480290.16977053706894735No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA467880.16538391156138807No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT465520.1645497104173236No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT454570.16067915849888897No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA441670.15611933021141802No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA437760.15473724272273498No Hit
AAGAGGTGCACAATCGACCGATCCTGA434580.15361319202861423No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG422960.14950581181927994No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC382120.1350698903262324No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA372980.13183912826828786No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG368140.13012830897283365No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT360800.12753380202476877No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA348530.12319666302575571No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG341630.12075768510455033No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT341350.12065871208745793No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGT306880.10847442673326231No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGGT306390.10830122395335062No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT304960.10779575461605734No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC297880.10529315118386398No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGA293780.10384390343358252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)