FastQCFastQC Report
Fri 30 Jun 2023
SRR1944966.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944966.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19923879
Total Bases616.3 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAGGTGCACAATCGACCGATCCTGA7443303.735868903841466No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG5469762.7453288589034295No Hit
CAAGAGGTGCACAATCGACCGATCCTGA2607491.3087260768849278No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG1841780.9244083443791242No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1374970.6901115992523343No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT1177990.5912453092091154No Hit
AAGAGGTGCACAATCGACCGATCCTGA820550.41184249312094295No Hit
TCAATATAGATGGATACGAATAAGGCGTCC796160.3996009010092864No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT771590.3872689650444073No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA741620.3722267134828514No Hit
TCCGATATAGTGTAACGGCTATCACATCACG718950.36084840707976595No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT633050.3177343126807787No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC583750.29299013510371147No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA537290.269671382766378No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC509370.2556580473109679No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC432080.216865400557793No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA397400.1994591515035802No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA388130.1948064430626185No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT357150.17925726210242493No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAA331520.16639330122412407No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG320790.16100780375146828No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG305450.15330849981572364No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA302400.15177767341389697No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT278920.13999281967131No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA273390.13721725573619475No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT245490.12321395848669829No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG229020.11494749591683426No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG225990.11342670772092121No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT220470.11065616288876277No Hit
ATGTACACGGAGTCGACCCGCAACGCGA212330.10657061308192045No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCT211710.1062594286985983No Hit
ACAAGAGGTGCACAATCGACCGATCCTGA200240.10050251760713866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)