FastQCFastQC Report
Fri 30 Jun 2023
SRR1944968.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944968.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16294211
Total Bases509.1 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAGGTGCACAATCGACCGATCCTGA4336832.6615771699531816No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG1632681.002000035472721No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC1631111.0010365030868937No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1521120.9335340017384088No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1469640.9019399589216073No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1239520.7607118871849641No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA1004820.6166730012272457No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC962640.5907865069379548No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT844170.5180797032762127No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG636510.39063566809095573No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG608160.37323685080547936No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC571380.35066441695151734No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA558920.34301752935444374No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG535620.3287179722908952No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT526530.323139303891425No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT522320.32055556418165937No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC511500.313915169013093No Hit
TCAATATAGATGGATACGAATAAGGCGTCC507100.31121482347319546No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA504450.3095884790003026No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC464280.2849355516508286No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA459130.2817749199393576No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG457230.2806088616380382No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT433370.26596562423304815No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC429150.26337574737432823No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT391590.24032461590192986No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA380120.23328530605133319No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT376910.23131528123699882No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC371070.22773118624768024No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG370250.2272279400334266No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG369240.22660808798904103No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA358510.22002292716106353No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT346250.2124987825430762No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA342270.21005619725925975No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC322900.1981685397347561No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG320270.19655446955977188No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC273870.16807809841176108No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA261780.16065828532599707No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT261280.16035142787828144No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG260900.16011821621801756No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG236900.14538905872766716No Hit
AACTATGCCGACTAGGGATCGGGTGGTG225690.13850931474988265No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA225350.138300651685436No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT219430.13466745950448292No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGGT217190.1332927381387169No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG215970.13254400596629073No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCT200950.1233260082369131No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG181810.11157950513835865No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAA176110.10808133023440043No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT172280.10573080218489866No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTG170650.1047304469053457No Hit
TTTGTGGCGTCGCTGAACCATAGCAGGCTAGC167660.10289543936800621No Hit
TAGTCAGAATCCATGCTAGAACGCGGTGATTT166790.102361507408981No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAT165950.10184598689681876No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGT164350.10086404306412873No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATA163050.10006621370006807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)