FastQCFastQC Report
Fri 30 Jun 2023
SRR1944972.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944972.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21873322
Total Bases686.5 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAGGTGCACAATCGACCGATCCTGA3545221.6207963289709721No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG2507931.1465702374792452No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1744770.7976703310087054No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT1593100.7283301548799949No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1488390.6804590541848193No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG1332650.6092581638948121No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1211670.5539487783337163No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1122610.5132325121899637No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1074470.4912239668030306No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC983040.4494241889732159No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG776970.3552135336370031No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT633680.2897045085332717No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA626900.2866048421908661No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA593900.27151797061278576No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGT561670.2567831260381939No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC530100.24235001889516372No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT527170.2410104875702008No Hit
TCAATATAGATGGATACGAATAAGGCGTCC523580.23936921881367634No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG514980.23543748864484326No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG505220.23097543208114432No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT503890.23036738543875504No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG492990.22538414603872242No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC492010.2249361116706461No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG477190.2181607347983082No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC468520.21419700217461252No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT464460.21234085979258202No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG449260.20539175530813286No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG449000.20527288904721466No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA427830.19559443234091284No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA426700.1950778212838452No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT418020.19110951688088348No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC411430.18809671434453348No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT386980.17691871403895576No Hit
TTCCTATAGTGTAGTGGTTATCACTTTCGGT381440.1743859483255447No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC362580.16576357262970848No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA356720.1630845099797827No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT355160.1623713124142734No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC344960.15770809756286677No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA336580.15387694653788758No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC308150.14087937808440804No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG300840.13753740744089993No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT294400.13459318159354122No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA291100.13308449443573317No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG285930.13072088455516725No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGG262090.1198217627848207No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA254450.11632892342553179No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA250360.11445906570570305No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC250290.11442706325084046No Hit
TTCCTATAGTGTAGTGGTTATCACTTTCGG240800.11008844472732582No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA237430.10854775511465518No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG235910.10785284466621027No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT234300.10711678820437061No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT229220.10479432433719944No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT223930.10237585310544049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)