FastQCFastQC Report
Fri 30 Jun 2023
SRR1944973.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944973.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22148194
Total Bases693.7 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAGGTGCACAATCGACCGATCCTGA4934522.227955922726702No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG2452101.1071331594801817No Hit
CAAGAGGTGCACAATCGACCGATCCTGA2078070.938257087688504No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1551870.7006756397383913No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1405160.6344354758676938No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1331310.6010918994117533No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC1278890.5774240554331428No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1043450.47112193436629646No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT892130.4028003366775639No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG801570.3619121270113491No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG745330.33651953743948604No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG667710.3014737905943934No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG578520.26120414152052307No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC561610.25356920749384804No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA551470.24899095610233504No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA539950.24378962907765753No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT539250.24347357622025523No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG524820.23695837231694827No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT519570.2345879758864312No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT508220.22946340455569425No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA478040.21583701136083602No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG473810.21392714909396227No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC472070.21314153199127658No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG467520.21108718841816176No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT457960.20677080939421064No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC456050.20590843659758443No Hit
TCAATATAGATGGATACGAATAAGGCGTCC456040.2059039215567644No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA451700.2039443938408703No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC447270.20194423075759585No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA445040.20093737665472858No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG436610.19713119724344116No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT430250.19425963128190046No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA370080.1670926306677646No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA365830.16517373831925078No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT343280.1549923212700774No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG341930.15438279075937297No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC335720.15157895041013275No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC325610.14701424414107986No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT320850.14486508471074436No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGT318810.14394401638345772No Hit
AACTATGCCGACTAGGGATCGGGTGGTG311150.1404854951153128No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA303770.13715339499012877No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGGT297950.13452564123286984No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA297120.1341508928448071No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC294030.13275574523141706No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA290290.1310671199647249No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG286050.12915274265703108No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC283730.12810525318678354No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT257340.11619006046271764No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC255360.11529608238035119No Hit
TCTACTTGCGTGCCTTGTTGTAGACGGCCTTGG250970.1133139794603569No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAT242700.10958004070218998No Hit
TTTGTGGCGTCGCTGAACCATAGCAGGCTAGC240220.10846031057882191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)