FastQCFastQC Report
Fri 30 Jun 2023
SRR1944974.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944974.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14882329
Total Bases464.8 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAGGTGCACAATCGACCGATCCTGA2975701.999485430002253No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG2020781.3578385479853323No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1445720.9714339738088038No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1067830.7175153835128897No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT917980.6168254982133509No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG787810.5293593496017995No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA785150.5275719949478338No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC763750.5131925251753271No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC691480.4646315774903243No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT689110.4630390848099112No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG545220.36635394903579943No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA505300.3395301904695159No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT475010.31917719330086036No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC467610.31420485328606834No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT461130.3098506960839261No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA428620.288005996910833No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGT386190.2594956743665592No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC353340.23742251632792155No Hit
TCAATATAGATGGATACGAATAAGGCGTCC338980.22777348894786562No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA336550.22614067999706228No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG324420.21799007399984235No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT318780.21420034458316303No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT313190.2104442120584755No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG307960.20692997715612924No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG305870.20552562707087044No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA294670.19799992326469867No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA289940.1948216572822708No Hit
TTCCTATAGTGTAGTGGTTATCACTTTCGGT278550.1871682852865301No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT273730.18392954489851687No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC270760.18193388951420172No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC266640.17916550561407424No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG262170.17616194347000394No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG260650.17514059795345205No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG260550.17507340416946837No Hit
AACTATGCCGACTAGGGATCGGGTGGTG258720.1738437579225671No Hit
AAGAGGTGCACAATCGACCGATCCTGA257940.17331964640749442No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT246870.16588129452050146No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT246190.16542437678941246No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC239750.16109709710086373No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC227950.15316823059078993No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT219820.14770537595291705No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGG216660.14558205237903288No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG209360.1406769061482245No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA209010.14044172790428164No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC203330.13662512097400883No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA198970.13369547199232054No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG198900.133648436343532No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA181860.12219861555271357No Hit
TTCCTATAGTGTAGTGGTTATCACTTTCGG181200.1217551365784213No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA180690.12141244828010454No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA178810.12014920514121144No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT177500.11926896657102529No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC170330.11445117225939569No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG161810.10872626186398647No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAA160650.10794681396977582No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT159470.10715392731876845No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC155560.10452665036500672No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAT150150.10089146665148982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)