FastQCFastQC Report
Fri 30 Jun 2023
SRR1944975.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944975.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11921107
Total Bases363.3 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAG7294456.118936773237587No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA3692733.0976401772083753No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG2117281.7760766680476905No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1423081.193748198049057No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1175160.985780934606157No Hit
AAGAGGTGCACAATCGACCGATCCTGA1026520.8610945275468126No Hit
TCAATATAGATGGATACGAATAAGGCGTCC788910.6617757897819389No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC383230.32147182304462163No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT361480.30322687314189867No Hit
TCCGATATAGTGTAACGGCTATCACATCACG360460.3023712479050813No Hit
AACTATGCCGACTAGGGATCGGGTGGTG353710.29670902207320177No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA328770.2757881461847461No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG323030.27097315710697No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC316050.26511799617267084No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT292370.24525406910616607No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA287380.24106821623193214No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA206390.1731298947320916No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC202280.16968222833668048No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG182180.15282137808175028No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA171190.143602435579179No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT159230.13356981025335984No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT148900.12490450760990568No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG145130.12174204962676705No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT142990.11994691432599337No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT141590.11877252674604799No Hit
GTTTCTTTACTTATTCAATGAAGCGGAG136850.11479638593966147No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT136540.1145363429755307No Hit
AAGTTTCTTTACTTATTCAATGAAGCGGAG131120.1099897853445993No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGC130700.10963746907061568No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA129880.10894961348807623No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGT129700.10879862079922611No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG127130.10664278074175496No Hit
ACAAGAGGTGCACAATCGACCGATCCTGA126880.10643306867390756No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT124470.1044114443398587No Hit
AAATGATTAATAGGGACGGTCGGGGGCATCA122780.10299379076121035No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATA120890.10140836752828408No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG119980.10064501560131957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)