FastQCFastQC Report
Fri 30 Jun 2023
SRR1944976.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944976.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17430822
Total Bases545.5 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAGGTGCACAATCGACCGATCCTGA3382431.9404879471547585No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT1724790.9895058305339818No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1596480.9158948442018398No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC1371360.7867443084439735No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG1243290.7132710092501662No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1241110.7120203510769602No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1239990.7113778110980653No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1238030.7102533661349992No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1202610.6899330393024494No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG1085500.622747452759256No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA861730.49437140715452205No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT781530.4483609550943725No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC732890.4204563617252245No Hit
TCAATATAGATGGATACGAATAAGGCGTCC698590.4007785748715694No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT696760.39972871044176805No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC587270.33691469054069856No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG562640.322784547969109No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA553130.3173286951126No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC547810.3142766302128494No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG532140.30528680747241865No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC505650.2900895895787359No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG492900.2827749603547096No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG490140.28119155826386155No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG466530.2676465860301941No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT461290.2646404168432217No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT454240.2605958571546425No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG450200.2582781236593432No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC423930.24320711897580044No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGT406860.23341412126175118No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT401280.23021289529547143No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT396660.22756241788253012No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC390960.22429234834708311No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT381140.21865864960355857No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA373950.2145337724176175No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTG364940.20936476776597227No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT358680.20577342824107778No Hit
TTCCTATAGTGTAGTGGTTATCACTTTCGGT346150.19858501222719155No Hit
AACTATGCCGACTAGGGATCGGGTGGTG344670.19773594154079482No Hit
AGAGATGTATTTATTAGATAAAAAATCAATG317660.18224040151405368No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC312190.17910228215284396No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA304080.17444960426995354No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG296690.17020998780206692No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT293300.16826515697309052No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA273770.15706086609111147No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA270740.155322565969637No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC267590.15351542227899523No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG264440.15170827858835342No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA258200.14812841299165352No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA257250.14758340140241236No Hit
TTCCTATAGTGTAGTGGTTATCACTTTCGG250000.1434241024318876No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGG243150.13949428202525388No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA238460.1368036458636317No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC232360.1333040977642936No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG228760.13123879068927444No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG215300.12351683701434162No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAT208490.119609964464097No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGA206190.11829046272172362No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGGT203970.11701685669212845No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGC202500.11617352296982895No Hit
TTTGTGGCGTCGCTGAACCATAGCAGGCTAGC201940.11585225298038153No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG201210.1154334546012804No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT199910.11468764926863462No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG184590.10589862027160853No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT181120.10390789372985393No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTGA180440.1035177801712392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)