FastQCFastQC Report
Fri 30 Jun 2023
SRR1944977.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944977.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19278040
Total Bases603.3 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAGGTGCACAATCGACCGATCCTGA3354971.7403065871841743No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG1839990.9544486887671153No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1757250.9115293878423325No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1513000.7848308230504761No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1367070.7091332936335851No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG1287390.6678012910026123No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC1263140.6552222113866347No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1145130.594007482088428No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA1099570.5703743741583689No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT958570.4972341586592828No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA920990.47774047569151223No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC871700.4521725237627891No Hit
TCAATATAGATGGATACGAATAAGGCGTCC828830.4299347859014713No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG655110.3398218906071364No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC644460.33429747007475863No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT623080.32320713101539367No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC611230.3170602405638748No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT577110.29936134586296115No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT526350.2730308682832902No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGT524000.2718118646916388No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC512890.26604883069025687No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG509080.2640724886969837No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG491740.25507779836539396No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG451510.23420949432618668No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG434150.2252044294959446No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC419870.2177970374581648No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA419760.2177399777155769No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG409290.2123089276710703No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC407080.21116254556998534No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT401020.20801907247832246No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT386730.2006064931912165No Hit
TTCCTATAGTGTAGTGGTTATCACTTTCGGT379240.1967212434459105No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA358200.18580727086363552No Hit
AACTATGCCGACTAGGGATCGGGTGGTG344760.17883560776925456No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTC340910.17683851677867665No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGG333470.1729792032800015No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAT325340.16876196957782016No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA314690.16323754904544238No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT314540.16315974030554975No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC307270.15938861004541957No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT302740.1570387861006617No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG301400.15634369469095408No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG299990.1556122925359632No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT297190.15415986272463383No Hit
TTCCTATAGTGTAGTGGTTATCACTTTCGG295520.15329359208716237No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA294890.15296679537961327No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA294840.1529408591329824No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA291920.1514261823297389No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT254760.13215036383366774No Hit
TTTGCTCCACACAATATAGATGGATACGAAT254210.13186506512072804No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC250430.1299042848754334No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG245570.1273832817029117No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC236440.12264732306811273No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT232520.12061392133225163No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTG232100.1203960568605522No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA230440.11953497347240694No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT219170.11368894348180625No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA208250.1080244672176217No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG205380.1065357266610091No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT203580.10560202178229738No Hit
TAGTCAGAATCCATGCTAGAACGCGGTGATTT199430.1034493133119342No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGC195040.1011721108577428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)