FastQCFastQC Report
Fri 30 Jun 2023
SRR1944978.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944978.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17133451
Total Bases519.1 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGAGGTGCACAATCGACCGATCCTGA10289376.005427628094305No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG4879362.847855928148976No Hit
CAAGAGGTGCACAATCGACCGATCCTGA4176152.4374248947278634No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC2666551.5563414515849725No Hit
AAGAGGTGCACAATCGACCGATCCTGA2062771.2039430935425677No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG1118870.6530324801465858No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1066970.622740859386705No Hit
TCAATATAGATGGATACGAATAAGGCGTCC973790.5683560188779249No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA938660.5478522686410344No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC700490.4088434956857203No Hit
TCCGATATAGTGTAACGGCTATCACATCACG612760.3576395671835172No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA610640.35640222159563767No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC559400.3264958121980213No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT494520.2886283679802744No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT454000.2649787249515582No Hit
ATGTACACGGAGTCGACCCGCAACGCGA433430.2529729708276517No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGC430010.2509768755868272No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA395880.23105677892912524No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT377750.22047513953843859No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC323060.18855512529262203No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG322040.18795979864184978No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG317240.18515826146174522No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG307080.17922834109719052No Hit
ACAAGAGGTGCACAATCGACCGATCCTGA272610.1591098022225645No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA271080.15821681224640616No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG259550.1514872864783633No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG250630.14628109655200228No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC247020.14417410713113196No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCT237230.138460138590877No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT229030.1336741792415317No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT227260.1326411124063681No Hit
TAGTCAGAATCCATGCTAGAACGCGGTGATTT226630.13227341065147938No Hit
GTTTCTTTACTTATTCAATGAAGCGGAG215920.12602248081837103No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT196190.11450699570098283No Hit
TTTGTGGCGTCGCTGAACCATAGCAGGCTAGC195380.11403423630184019No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGGT191070.11151868937553794No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC190010.11090001658159818No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT188070.10976772863797257No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAGC185350.10818019090257998No Hit
TTTACCCAATCCTAATTCAGGGAGGTAGTG174550.10187673224734468No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT173670.10136311709765884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)