FastQCFastQC Report
Fri 30 Jun 2023
SRR1944979.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944979.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40491503
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length25-48
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA17127084.229796063633399No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG16241964.011202053922276No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA14956793.6938095382628795No Hit
AAGAGGTGCACAATCGACCGATCCTGA9537702.3554818402270716No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC4584501.1322128496934283No Hit
ATGTACACGGAGTCGACCCGCAACGCGA2779920.686544038634476No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG2433830.6010717853570414No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1687510.41675657236037894No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1462910.3612881448238659No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC1421610.3510884740435543No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA1371150.3386266002524036No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGC1363310.3366903915619037No Hit
TCAATATAGATGGATACGAATAAGGCGTCC1141500.28191099747519865No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA994290.2455552217955456No Hit
GTTTCTTTACTTATTCAATGAAGCGGAG948950.23435781082268048No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT918860.2269266221113106No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT804880.19877750648080417No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA706600.17450574753918124No Hit
AAGAGGTGCACAATCGACCGATCCTG687010.16966769546687363No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC640720.15823566736952194No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGC617840.15258509915030816No Hit
ATTTTCTTTACTTATTCAATGAAGCGGAG568630.14043193210190294No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA559420.13815738082135404No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT527540.1302841240543726No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC525500.12978031465021192No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG466600.11523405293204356No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCA450560.11127272800913318No Hit
TAAGAGGTGCACAATCGACCGATCCTGA419960.10371558694672312No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCT411610.10165342590518311No Hit

[OK]Adapter Content

Adapter graph