FastQCFastQC Report
Fri 30 Jun 2023
SRR1944980.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944980.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20302158
Total Bases611.2 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAG17478938.609395119474492No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG4517512.2251378400266613No Hit
ATTTTCTTTACTTATTCAATGAAGCGGAG3622511.7842980041826095No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA3294391.6226797171019949No Hit
CAAGAGGTGCACAATCGACCGATCCTGA3283311.617222169190093No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT1888360.9301277233681268No Hit
AAGAGGTGCACAATCGACCGATCCTGA1860740.9165232582664365No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1792840.8830785377593849No Hit
GTTTCTTTACTTATTCAATGAAGCGGAG1359210.6694904058967525No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA1272560.626810213968387No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC944940.46543820612567394No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT834740.4111582620921382No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT788680.38847101869663314No Hit
TCAATATAGATGGATACGAATAAGGCGTCC783190.3857668726644724No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT651460.3208821446468893No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC613080.3019777503455544No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA586200.2887377785159588No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG574530.2829896211033329No Hit
TAATGATTAATAGGGACGGTCGGGGGCATCA460730.22693646655690494No Hit
TCCGATATAGTGTAACGGCTATCACATCACG451200.22224238428249846No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT441290.21736112978728664No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGT403800.19889511252941683No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG391730.19294993172646965No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGC371940.1832021994903202No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA345240.17005088818636915No Hit
AAATGATTAATAGGGACGGTCGGGGGCATCA328770.161938450089887No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT322320.158761447920955No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG318180.1567222558311289No Hit
AAGTTTCTTTACTTATTCAATGAAGCGGAG302310.1489053528201288No Hit
AGGTTCTTTACTTATTCAATGAAGCGGAG291260.14346258166250112No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA287930.14182236193807576No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA279560.1376996474956012No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAG268730.13236523920265028No Hit
TAATGATTAATAGGGACGGTCGGGGGCATC247920.12211509732118132No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA232460.1145001432852606No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA231990.11426864080163301No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT230960.1137613055715555No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAA230410.11349039840986362No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC229010.11280081654373884No Hit
TAATGATTAATAGGGACGGTCGGGGGCAT228070.11233781157648365No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC226190.11141180164197324No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT221850.10927409785698643No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG208440.10266888869646272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)