FastQCFastQC Report
Fri 30 Jun 2023
SRR1944981.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944981.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27554640
Total Bases841.8 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA7612252.7626018703202075No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG2370660.8603487470712736No Hit
TCCGATATAGTGTAACGGCTATCACATCACG2304400.8363019803561215No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT2061030.7479792876989139No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1782210.6467912482253442No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1768570.6418410837521376No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1749780.6350219055665398No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC1708810.6201532663827217No Hit
CAATATAGATGGATACGAATAAGGCGTCC1288330.4675546477834586No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1195310.43379626806955196No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1091020.39594783310542253No Hit
AAGAGGTGCACAATCGACCGATCCTGA1034780.37553747753554395No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA995320.3612168404305046No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG857570.3112252600650925No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT744350.27013599161520524No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG729870.2648809783034727No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA725850.26342205886195574No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT688730.24995064352138155No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC668260.2425217676587319No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT622630.22596194325166288No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG622310.22584581036079587No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG613680.22271385146022593No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT518820.18828770762383396No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCT517440.18778688453196996No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT517200.18769978486381966No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC503010.1825500169844353No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG484200.1757235804931583No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT473220.17173877067528373No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT454820.16506112945043014No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT451750.16394697952867465No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT440500.15986418258413104No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA436170.15829275940458667No Hit
TCAATATAGATGGATACGAATAAGGCGTCC433800.1574326501816028No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC431410.15656528265293976No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT335010.12158024927925025No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGT330570.11996890541847034No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC307640.11164725795728052No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT302160.10965848220118282No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGG300390.1090161221485746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)