FastQCFastQC Report
Fri 30 Jun 2023
SRR1944982.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944982.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24185766
Total Bases738.7 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA6904822.8549106114728806No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG3312281.3695162683704127No Hit
TCCGATATAGTGTAACGGCTATCACATCACG2130990.8810926228261697No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1891760.782179071773042No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC1810200.7484567575821249No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1701250.7034096005063474No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1552860.642055331222505No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1480220.6120211367297608No Hit
CAATATAGATGGATACGAATAAGGCGTCC1329480.54969522156131No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1130120.4672665732398139No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1047340.43303983012156816No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA919590.38021950596892407No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG850420.35162003965472916No Hit
AAGAGGTGCACAATCGACCGATCCTGA779420.3222639299495414No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG757460.31318420925762697No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG692150.286180722992193No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT621470.25695692251384555No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT616290.25481516690436845No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA593710.24547909708545101No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC550320.22753879285857642No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG536180.22169237889757143No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT524990.21706569062150027No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT509330.2105908078330039No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC471050.19476331657223508No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT466800.19300608465326258No Hit
TCAATATAGATGGATACGAATAAGGCGTCC466230.19277040884295332No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC461170.19067826919354136No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA447710.18511301233957195No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT402470.16640779539502698No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT396560.1639642093618205No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG373630.15448342632604647No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT320230.13240432409707428No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC289640.11975638894381099No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGC270470.11183023932341031No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGT265460.10975877299069213No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT260680.10778240391476542No Hit
CTTGCGTGCCTTGTTGTAGACGGCCTTGG260640.10776586526141035No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA259320.1072200897006942No Hit
TTTACCCAATCCTAATTCAGGGAGGTAGTG254940.10540910715831782No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGG248390.1027009026714308No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT248090.10257686277126803No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAA245790.1016258902033535No Hit
AGTCAGAATCCATGCTAGAACGCGGTGATTT244590.10112973060270242No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA243660.10074520691219786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)