FastQCFastQC Report
Fri 30 Jun 2023
SRR1944983.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944983.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24852461
Total Bases755.5 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTATGCCGACTAGGGATCGGGTGGTGT3045761.2255365776451677No Hit
CAAGAGGTGCACAATCGACCGATCCTGA2461810.9905699077447501No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC2393020.9628905563919807No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG2024860.8147523096404818No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1477290.5944240290730162No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1423250.5726797036317651No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1334330.5369005508146658No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG1268770.5105208695428594No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC1191790.47954606990430443No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT1128270.4539872328941589No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT1090980.4389826826405642No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA1062680.4275954803832104No Hit
CAATATAGATGGATACGAATAAGGCGTCC851430.3425938380911251No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG844580.3398375718203521No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT795650.32014938077963384No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT764980.3078085506300563No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGT722200.2905949636134627No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT720460.2898948317432225No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG707670.28474846012231947No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG693470.2790347402617391No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT634980.25549984768108075No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG584640.23524430840068514No Hit
AAGAGGTGCACAATCGACCGATCCTGA577160.2322345461079287No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGT577150.232230522361548No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA565790.2276595464730837No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCT547230.22019147319052226No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT543960.2188757081240365No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC537670.2163447716505822No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT478010.19233910074338312No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT463820.1866294046291834No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC448890.18062195128281258No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT413440.16635777036326502No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT396030.15935242791448298No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG383140.15416581882977304No Hit
TGCTTGGTGGGGCTTGCTCTGCTAGGCG376450.15147393250109115No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA375840.15122848397186903No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGG372550.14990467141262187No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGC350300.14095183571558567No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGT332600.1338298046217636No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGA331180.13325843263570558No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG323770.13027683656761396No Hit
CTTGCGTGCCTTGTTGTAGACGGCCTTGG316060.12717452810810165No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC315430.12692103208611816No Hit
AAATATAGATGGATACGAATAAGGCGTCC313910.1263094226362532No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG310620.12498561007700607No Hit
TCAATATAGATGGATACGAATAAGGCGTCC297910.11987142842714853No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA294900.11866028076656071No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT288950.11626615167004989No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCT286220.11516766890812141No Hit
GGTCCTATAGTGTAGTGGTTATCACTTTCGG270660.1089067195397671No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG269810.10856470109740841No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGC260650.10487894941269599No Hit
ACTTGCGTGCCTTGTTGTAGACGGCCTTGG260640.1048749256663153No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCT257720.10369999172315368No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT248650.10005045375586748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)