FastQCFastQC Report
Fri 30 Jun 2023
SRR1944984.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944984.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33131925
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length25-47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA10687603.2257709143069713No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG5203631.5705788299351757No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT3639961.0986261739998506No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA3593191.0845098798213504No Hit
TCCGATATAGTGTAACGGCTATCACATCACG3194820.9642723747563716No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC2597240.7839085715665479No Hit
AACTATGCCGACTAGGGATCGGGTGGTG2350780.7095211038899792No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC2113290.6378409947505314No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1844870.5568254787489709No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1784790.5386919112004509No Hit
CAATATAGATGGATACGAATAAGGCGTCC1484480.44805123759033016No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG1165120.3516608226053874No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1092110.3296246746906496No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT934360.2820119869280158No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT896180.27048835828283446No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG824780.248938146515785No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT818580.24706683961164344No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT782090.23605329300968778No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT781340.23582692523902551No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT769370.23221409561925543No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT767420.23162553941553352No Hit
ATGTACACGGAGTCGACCCGCAACGCGA752700.2271826946366684No Hit
TCAATATAGATGGATACGAATAAGGCGTCC746870.2254230624993869No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT738790.22298432705011856No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG729090.2200566372162197No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC694790.2097040845045979No Hit
AAGAGGTGCACAATCGACCGATCCTGA679570.2051103278786246No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC674260.20350764406233565No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA673910.2034020057693599No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT637710.19247598803872698No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG635100.19168822819682224No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT606970.18319792767851553No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC575390.17366633541516227No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG561790.16956153317381953No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT548910.16567404399231256No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT541060.16330472799271398No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT509330.15372786217522827No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA503990.1521161236481128No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT502650.15171167989786288No Hit
CTTGCGTGCCTTGTTGTAGACGGCCTTGG474520.14322137937955612No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCT466160.1406981332959072No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC458430.1383650361396146No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGC418400.12628303366013294No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCT412480.12449623739037197No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG402830.12158363874118393No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG397830.12007452027010203No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA396780.11975760539117482No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA388880.11737319820686544No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA372570.1124504537541963No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGG365570.11033768789468165No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAT358200.10811324726830691No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG357100.1077812412046689No Hit
ATCTTTGAACGCACATTGCGCCCCTTGGT352630.10643208929152169No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAT352370.10635361513102544No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG345250.10420463042820481No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG343180.10357985538117691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)