FastQCFastQC Report
Fri 30 Jun 2023
SRR1944985.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944985.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24862035
Total Bases761.8 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA5096692.0499890696799357No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG2708261.0893154964989793No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1850600.7443477575347311No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1612360.6485229386894515No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC1459490.5870356147435236No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1302670.5239595230237589No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1230840.4950680827213058No Hit
AACTATGCCGACTAGGGATCGGGTGGTG954530.3839307602937571No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC930700.3743458650910917No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT806370.3243378910857458No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT731980.29441676837797065No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT679950.27348927792917993No Hit
CAATATAGATGGATACGAATAAGGCGTCC660680.2657385045109944No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA624770.2512947954582157No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG541340.21773760675664725No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT521340.20969321296506904No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC448860.18054032986438961No Hit
AAGAGGTGCACAATCGACCGATCCTGA413060.16614086497746464No Hit
ATGTACACGGAGTCGACCCGCAACGCGA410090.1649462724994153No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT405000.16289897427945862No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT403070.16212269027857132No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG395240.15897331010916846No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG378150.1520993756142649No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCT372660.14989118951847666No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC369810.14874486340317677No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT342430.13773208830250622No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA341240.1372534468719073No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA340280.13686731596991156No Hit
TGCGTGCCTTGTTGTAGACGGCCTTGGT320100.12875052263420916No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT307850.1238233314368675No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT300260.12077048399296356No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG297340.11959600249939314No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT296610.11930238212600054No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC292800.1177699251087049No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT292060.11747228253841652No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG291420.117214861937086No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT288360.11598406968697454No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGG283720.1141177703273284No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC281360.11316853185992218No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT275040.11062650342178344No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT270860.10894522511934361No Hit
CTTGCGTGCCTTGTTGTAGACGGCCTTGG269740.10849473906701522No Hit
CGGTGCCCGAGTTGTAATTTGGAGAGGGC268710.10808045278674897No Hit
TCAATATAGATGGATACGAATAAGGCGTCC257500.10357157006656936No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG250060.10057905557610228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)