FastQCFastQC Report
Fri 30 Jun 2023
SRR1944986.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944986.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30238345
Total Bases927 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA6513632.154096065773441No Hit
TCCGATATAGTGTAACGGCTATCACATCACG3653851.2083498617401185No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT3349191.1075969931555447No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC2551920.8439350764732659No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG2455580.8120748671926324No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC2347310.7762693361690265No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA2120760.7013479077641319No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1736320.5742113200970489No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1660620.5491768812082803No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1224980.4051081499334703No Hit
CAATATAGATGGATACGAATAAGGCGTCC1205230.39857670781916144No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG1156120.3823357396047965No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGA1053910.34853428651601137No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT973000.32177686973278463No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC896790.2965737708197985No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG820970.27149964721944936No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG780110.25798700292625143No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT753790.24928282285290415No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT706080.23350484294031304No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG672840.22251217783248392No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA657200.21733993708981098No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC636870.21061668553619584No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT626090.20705167561253765No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT619160.2047598835187574No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT582120.19251053587754224No Hit
TCAATATAGATGGATACGAATAAGGCGTCC578280.19124062510696271No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT567490.18767230812400612No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA565190.18691168448537776No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT564050.18653467972536197No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG502820.16628555564135536No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC479530.15858341453541852No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT456870.15108961816527988No Hit
AAGAGGTGCACAATCGACCGATCCTGA445590.1473592552767025No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG442890.14646634926613875No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT431500.14269960872527912No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGG428980.1418662297820863No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT416120.13761335152436419No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGT404650.1338201545091175No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT402040.1329570120322392No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGT391550.12948790682823413No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG377880.12496715676734292No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGC372020.12302922001848977No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGT352550.11659037556453569No Hit
CCGATATAGTGTAACGGCTATCACATCACG350810.11601494724661685No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC346260.11451023526585201No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG342400.1132337103766757No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC338740.11202332667346708No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA329770.10905689448281644No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGG311060.10286938653553956No Hit
CTAGAGGTGCACAATCGACCGATCCTGA307240.1016060898835568No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)