FastQCFastQC Report
Fri 30 Jun 2023
SRR1944987.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944987.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5157425
Total Bases155.1 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA3187866.18110782027853No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG1840973.569552635278264No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1263212.449303673829479No Hit
AAGAGGTGCACAATCGACCGATCCTGA720561.3971313203779017No Hit
TCCGATATAGTGTAACGGCTATCACATCACG404210.7837438256494278No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG389600.7554157355657135No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC368850.7151824796288846No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT308920.5989810806749493No Hit
AACTATGCCGACTAGGGATCGGGTGGTG257890.5000363553517502No Hit
TCAATATAGATGGATACGAATAAGGCGTCC214330.41557560216580947No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT212360.41175586654192736No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC174320.33799812891122993No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT173940.3372613271157603No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA146880.28479328346994864No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC145990.28306761610687503No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG135990.2636780951734635No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT135950.26360053708972986No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA131330.2546425784184937No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT127470.24715822333819687No Hit
CAATATAGATGGATACGAATAAGGCGTCC124700.2417873260396419No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC122150.2368429982016219No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG111420.21603804224007137No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT92910.1801480389923266No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG89790.1740985084611022No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG81140.15732657285370122No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT79180.15352622675075256No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA76350.1480389923265971No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAA76120.14759303334512863No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGT75500.14639088304725711No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT73240.1420088513163061No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT69870.13547458276174643No Hit
ACAAGAGGTGCACAATCGACCGATCCTGA67790.13144156240759683No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG64930.1258961594206411No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGT63430.12298773128062937No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC61050.11837302529847744No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC59090.11457267919552877No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG56810.11015186842271095No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC56150.10887216004110578No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT55890.10836803249683709No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC55260.10714649267803215No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT53680.10408294837055314No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT53290.10332675705415008No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGT51690.10022443370480424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)