FastQCFastQC Report
Fri 30 Jun 2023
SRR1944988.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944988.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31017570
Total Bases946.2 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAG13267284.277343454048786No Hit
CAAGAGGTGCACAATCGACCGATCCTGA11237813.623046550713031No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA5317521.714357378737277No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT5290251.7055655875041147No Hit
AAGAGGTGCACAATCGACCGATCCTGA3675551.1849896687587067No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG2567940.8278985104248978No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC2007040.6470655180273631No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT1178110.37982021157685786No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1110030.35787136129619435No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT1101820.3552244743866138No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1065190.3434150386377785No Hit
AACTATGCCGACTAGGGATCGGGTGGTG998380.32187563371340827No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA986890.31817128163166875No Hit
TCAATATAGATGGATACGAATAAGGCGTCC823050.26534960669065955No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA694790.22399884968422734No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT681610.2197496451204914No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA609660.19655311489584776No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC604310.19482828603272273No Hit
ATGTACACGGAGTCGACCCGCAACGCGA588780.18982144636088516No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAA556270.1793402900356153No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT536880.17308899439898098No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA474800.15307453162836418No Hit
CAATATAGATGGATACGAATAAGGCGTCC473790.15274890973083965No Hit
GTTTCTTTACTTATTCAATGAAGCGGAG454410.1465008380733887No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT424790.13695141173212472No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG412530.13299881325326257No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT389830.12568038050691913No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT364140.11739797798473574No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA348800.11245239391738296No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA344960.11121438591095306No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG338740.10920907085887128No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT335330.10810969395732807No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC334160.10773248839286895No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC330860.10666857526234325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)