FastQCFastQC Report
Fri 30 Jun 2023
SRR1944989.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944989.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25640547
Total Bases775.3 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAG12877295.022236850095281No Hit
CAAGAGGTGCACAATCGACCGATCCTGA10763314.197769259758772No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA2951041.1509270843558836No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC2383470.9295706522953664No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG2111940.8236719754847663No Hit
AAGAGGTGCACAATCGACCGATCCTGA2036780.7943590282999813No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT1396810.5447660691482128No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1203400.4693347610719849No Hit
AACTATGCCGACTAGGGATCGGGTGGTG1060410.41356762006676373No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC780710.30448258377639137No Hit
TCAATATAGATGGATACGAATAAGGCGTCC749030.2921271531375676No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT710060.27692856942560545No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT651100.25393374018112796No Hit
CAATATAGATGGATACGAATAAGGCGTCC632930.24684730789869655No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT503400.19632966488585443No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA495070.19308090424123947No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG493530.19248029302963No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT452230.1763729923546483No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT449300.1752302710234692No Hit
ATTTTCTTTACTTATTCAATGAAGCGGAG422440.1647546754755271No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTG397210.15491479179441842No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC381410.1487526767662172No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG380580.1484289707235965No Hit
AGGTTCTTTACTTATTCAATGAAGCGGAG375480.1464399335942404No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA370460.14448209704730558No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA359010.140016513688261No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT340380.1327506780569073No Hit
TTTAAGCCATGCATGTCTAAGTATAAGCAA337860.13176785971063723No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC332730.12976712236287313No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA326480.12732957686121127No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC311060.12131566459951108No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGT302520.11798500242604028No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTG291000.1134921185573771No Hit
AAACTATGCCGACTAGGGATCGGGTGGTG288320.11244689904626451No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT276840.10796961546881195No Hit
TTTGCTCCACACAATATAGATGGATACGAAT265440.1035235324737807No Hit
TTTACCCAATCCTAATTCAGGGAGGTAGTG257880.10057507743497048No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA257190.10030597241158701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)