FastQCFastQC Report
Fri 30 Jun 2023
SRR1944990.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944990.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18522847
Total Bases574.6 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA4513912.4369417940989306No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1832790.9894753220171822No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG1726740.9322217043632656No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1717130.9270335170397942No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT1502080.8109336539895838No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC1168380.6307777632671694No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT1092390.5897527523711663No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT897880.4847418973983859No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC830910.44858654827737876No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT786510.42461615107008116No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC679720.36696302679604276No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT671910.3627466123323267No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA658570.3555446956939179No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT585890.31630666711224253No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC565790.3054552035116416No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT529270.28573901193482837No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT527460.28476184033696333No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGA475960.25695833907174204No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG464860.25096573976991765No Hit
TCAATATAGATGGATACGAATAAGGCGTCC454170.24519448873059307No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC437420.23615160239675898No Hit
AAGAGGTGCACAATCGACCGATCCTGA420730.2271411084915834No Hit
ATGTACACGGAGTCGACCCGCAACGCGA410320.2215210221193319No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG393200.21227838247543696No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT378540.20436383240654094No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA366930.19809589746111922No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC366900.19807970124678997No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGT365310.19722130188733947No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG347390.1875467631946644No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTG318710.17206318229589654No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA317470.1713937387702873No Hit
CAATATAGATGGATACGAATAAGGCGTCC288600.15580758184743415No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA271240.14643537248890517No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT260700.1407451025212269No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA247550.1336457619069034No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT246810.13324625528678177No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT246690.13318147042946477No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC243220.1313081083053809No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA239750.12943474618129708No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT237680.12831720739257846No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG237410.12817144146361517No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGG233620.12612531972001928No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA215490.11633740752703946No Hit
ATTTTCTTTACTTATTCAATGAAGCGGAG205070.11071192241667817No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT203970.11011806122460549No Hit
TCAACGGGGCCTTTCCTTCTGGCTAACCTTGA201800.10894653505478936No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT198430.10712716031180305No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT193400.10441159504259795No Hit

[OK]Adapter Content

Adapter graph