FastQCFastQC Report
Fri 30 Jun 2023
SRR1944991.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944991.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17675011
Total Bases547.8 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA4422602.5021766605972693No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG2838481.605928279195979No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1768741.0007009330856993No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1624370.9190206444567417No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT1484000.8396034378705621No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1055570.5972103779737393No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT962670.544650297530225No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC847360.4794112999420481No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT820330.4641185230379772No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC788260.44597426276000623No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT781990.442426881657952No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT618520.34994037627473046No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG572960.32416387180749134No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT445370.25197721234798665No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC438430.2480507650037672No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG422480.23902672535819075No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC417430.23616958427918378No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT354340.200475122759471No Hit
TCAATATAGATGGATACGAATAAGGCGTCC350610.19836479875458066No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC342340.1936858766311376No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT340230.19249210085357232No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG338320.1914114791781459No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCA311890.17645816458049163No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT293000.16577075963347349No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGT290600.1644129104078068No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGA280480.15868731283957901No Hit
AAGAGGTGCACAATCGACCGATCCTGA274210.1551399317375248No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA271020.1533351238084095No Hit
AACTATGCCGACTAGGGATCGGGTGGTG256260.14498435107055946No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT255010.1442771379321914No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG251220.14213286769665942No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA244600.13838746691586218No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT242540.13722197966383162No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT239210.1353379638632191No Hit
CAATATAGATGGATACGAATAAGGCGTCC232200.131371912583251No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA225590.1276321695075607No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGG223790.12661378258831069No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT222860.12608761601336485No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC218530.12363782970205789No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA199760.11301831721632308No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT198370.11223189620645782No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA196640.1112531132229564No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA194000.10975947907472307No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT192930.10915410462828No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCA184310.10427716282609385No Hit
ATGTACACGGAGTCGACCCGCAACGCGA178110.10076938565978827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)