FastQCFastQC Report
Fri 30 Jun 2023
SRR1944992.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944992.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21756331
Total Bases661.8 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA11597185.330485181531757No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG4799872.206194601470257No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA4300171.9765143304723576No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1890420.8689056992192296No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1813400.8335045095609181No Hit
AAGAGGTGCACAATCGACCGATCCTGA1689320.7764728345050459No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1024380.47084225736407487No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG1000550.4598891237681574No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT984350.4524430153227582No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA826650.37995836706106373No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT692410.31825678695548437No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC640880.2945717271905819No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT614440.28241894278957236No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA596230.27404896533335515No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC520800.23937859742986997No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT503580.23146365993420492No Hit
TCAATATAGATGGATACGAATAAGGCGTCC494300.22719823484943302No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT486280.22351195153263664No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT481970.22153091897710145No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC477620.21953150096861462No Hit
AACTATGCCGACTAGGGATCGGGTGGTG409160.18806479824194622No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG404260.18581258025537487No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT403090.18527480575654046No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT402470.18498983123579063No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG333960.15350014669293274No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC323020.14847172531066932No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT313580.14413275841409107No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA305230.14029479511044393No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT303020.13927899883486788No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC302370.13898023522440434No Hit
CAATATAGATGGATACGAATAAGGCGTCC293890.13508251919866454No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC290890.1337036102272943No Hit
ACAAGAGGTGCACAATCGACCGATCCTGA284480.13075734139179992No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT277520.12755827257822103No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGT267310.12286538571232439No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT244960.11259251387561624No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGT241590.1110435394644437No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT240370.11048278314941982No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA239200.10994500865058544No Hit
ATCTTTGAACGCACATTGCGCCCCTTGGT227440.10453968548281417No Hit
TTTGCTCCACACAATATAGATGGATACGAAT226700.10419955460320952No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT221330.10173130754445683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)