FastQCFastQC Report
Fri 30 Jun 2023
SRR1944993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14979681
Total Bases449.7 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA9385306.265353714808747No Hit
ATGTACACGGAGTCGACCCGCAACGCG7533245.028972245804166No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA3742142.4981439858432233No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG3114622.0792298580991146No Hit
AAGAGGTGCACAATCGACCGATCCTGA1826851.219552005146171No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1772221.1830826036949653No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1067720.7127788635819414No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1055390.7045477136662657No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA835680.5578756984210812No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT743380.49625889897121306No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT740610.49440972741675876No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT686320.45816730009137047No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG638710.4263842467673377No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA620280.41408091400611263No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC609620.4069646075907758No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT581270.3880389709233461No Hit
TCAATATAGATGGATACGAATAAGGCGTCC552390.3687595216480244No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC536580.3582052247975107No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC532080.35520115548522024No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT509550.3401607817950196No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT455200.303878300212134No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA452510.3020825343343426No Hit
AACTATGCCGACTAGGGATCGGGTGGTG433870.2896390116718774No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG375600.25073965193250775No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG367930.24561938268244832No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC361500.2413269014206644No Hit
CAATATAGATGGATACGAATAAGGCGTCC343090.22903692007860516No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT302060.2016464836600993No Hit
ACTATGCCGACTAGGGATCGGGTGGTGT301150.20103899408805836No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC297360.1985089001561515No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT280640.18734711373359686No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGT246120.16430256425353784No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT235140.1569726351315492No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG232540.15523695064000362No Hit
ACAAGAGGTGCACAATCGACCGATCCTGA224930.15015673564744136No Hit
ATCTTTGAACGCACATTGCGCCCCTTGGT212830.14207912705217152No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAT207380.1384408653295087No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAT206460.13782670004788486No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGG195380.13043001383006753No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAA191760.12801340696106947No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCT170670.11393433545080166No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTG166700.11128407874640321No Hit
TTTGCTCCACACAATATAGATGGATACGAAT160810.10735208580209417No Hit
TTTGCGTGCCTTGTTGTAGACGGCCTTGGT159050.10617716091550948No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAT158280.10566313127762868No Hit
ATGTACACGGAGTCGACCCGCAACGC156370.10438807074730094No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC154100.10287268467199001No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGT153870.10271914335158407No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATA151430.10109027021336435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)