FastQCFastQC Report
Fri 30 Jun 2023
SRR1944994.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944994.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24446709
Total Bases749.4 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA9844644.026979664215744No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG7946523.250547957191293No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA3396721.3894385538765157No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT2551921.0438705676089162No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1874430.7667412411216578No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1668940.6826849372649709No Hit
AAGAGGTGCACAATCGACCGATCCTGA1477570.6044044619666394No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT1413560.5782209785374384No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG1410190.5768424698800971No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA916150.3747539188199115No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT746080.30518627272079857No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC593600.24281386913878675No Hit
TCAATATAGATGGATACGAATAAGGCGTCC500820.20486193049542986No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT495140.20253850937563828No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT479290.1960550191029803No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA474200.19397293926147688No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC423110.17307442077377366No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA388480.1589089148972976No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC368820.15086693264111745No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT357980.14643279796883907No Hit
CAATATAGATGGATACGAATAAGGCGTCC333880.13657462033028658No Hit
GCAAGAGGTGCACAATCGACCGATCCTGA314120.12849173277270162No Hit
ACAAGAGGTGCACAATCGACCGATCCTGA308190.12606604839939806No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT307860.12593106090476228No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC301420.12329675949429429No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG268760.10993708805549246No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGC268070.10965484147579946No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT246210.10071294258871409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)