FastQCFastQC Report
Fri 30 Jun 2023
SRR1944995.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944995.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27909322
Total Bases863.6 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGTGCACAATCGACCGATCCTGA6462282.315455746291508No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG3991671.4302282226705472No Hit
TCCGATATAGTGTAACGGCTATCACATCACG2968791.0637270228205473No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA2413220.864664501703051No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGC1654940.5929703344280451No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT1651150.5916123652161812No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC1606640.5756642887992764No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGT1137780.40767024007247477No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1070440.3835421010943942No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGC1034450.3706467681300176No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAT982030.3518645132260827No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT859330.3079007078710117No Hit
AAGAGGTGCACAATCGACCGATCCTGA847940.30381963417097696No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAG814440.2918164762296985No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCT801930.2873341029208807No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC773660.2772048708313301No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA717590.257114809166629No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT708650.2539115783608072No Hit
TCAATATAGATGGATACGAATAAGGCGTCC604450.21657638261509898No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGC525320.18822384864813269No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT511560.18329359631165532No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAT491310.176037956063569No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG484050.17343667467092178No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA466800.16725594408921865No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCT458210.16417811941114155No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGA452120.16199605278838375No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGC422180.15126845431787989No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTGAGT417840.1497134183338456No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG416060.14907563859845827No Hit
CAATATAGATGGATACGAATAAGGCGTCC384580.1377962531658777No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGA364700.13067318510997866No Hit
CAAGAGGTGCACAATCGACCGATCCTGAT362400.1298490876990849No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTG360670.12922922312480395No Hit
ATGTACACGGAGTCGACCCGCAACGCG345630.1238403426640031No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT343320.12301266222088805No Hit
CCAAGAGGTGCACAATCGACCGATCCTGA341210.12225664242219857No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT331950.1189387545852959No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGT326740.1170719947980105No Hit
CGGGGCCTTTCCTTCTGGCTAACCTTGAGT324090.11612249125937205No Hit
TCCGATGTAGTGTAACGGCTATCACATCACG323010.1157355237794741No Hit
TACTTGCGTGCCTTGTTGTAGACGGCCTTGG308870.11066911621858819No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT281350.1008086115456334No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGC280180.10038939677574396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)