FastQCFastQC Report
Fri 30 Jun 2023
SRR1944996.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944996.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18136118
Total Bases567.6 Mbp
Sequences flagged as poor quality0
Sequence length25-48
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGATGTATTTATTAGATAAAAAATCAATG7885564.347986708070603No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG5221542.879083605433092No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC3959492.183207012658387No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC2786741.5365691820046605No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT2722741.5012804835081024No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT2452321.3521747046418644No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT2377131.3107159977675487No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCT2247951.2394879653959023No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCA2043541.1267791707133799No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGC1937861.0685087073209383No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA1730270.9540465054318681No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGT1644680.9068533850518617No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG1601570.8830831382989459No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCAT1589300.8763176331340587No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1480240.8161834853522678No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG1443320.7958263174070659No Hit
AGAGATGTATTTATTAGATAAAAAATCAAT1401590.772816983215482No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATC1172980.6467646494139484No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCA1097180.6049695971320874No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT994910.5485793597064157No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCA987620.5445597563932921No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT947610.5224988059738032No Hit
TAGAGATGTATTTATTAGATAAAAAATCAAT922620.5087196719827253No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATG909640.5015626828188922No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGT871260.4804004914392374No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT849670.4684960695557892No Hit
AGATGTATTTATTAGATAAAAAATCAATG824130.45441367331200644No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACCA796700.4392891576907473No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT790500.4358705650238932No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG718500.39617077921526533No Hit
ATGTACACGGAGTCGACCCGCAACGCGA665040.3666936882523592No Hit
TAAGAGATGTATTTATTAGATAAAAAATCAATG658730.36321444313496415No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT646300.35636071622383575No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG625910.345117957437198No Hit
TACCATACGCGAAACTCAGGTGCTGCAATCT563830.3108879198955366No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG544500.30022963017774806No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGT537660.2964581505259284No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATC530380.2924440610719449No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCT513230.28298779264669544No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCT506980.2795416306841409No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAA491460.27098412129872557No Hit
TTTGATGATTCATAATAACTTTTCGAATCGC483220.2664407013672937No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAA476660.2628236097713965No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAAT471360.2599012644271503No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA461650.2545473072021256No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT461190.2542936696816816No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGT456380.2516415034352997No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC455140.2509577849019288No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACC450140.24820085533188524No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGC433010.2387556146249159No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGC429370.23674856989792412No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTG426630.23523777249354022No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTT413170.22781611809098287No Hit
TGGCATTAACGCGAACTCGGCCTACAATAGTGA387030.21340289029879492No Hit
GCCTTGTTGGCGCAATCGGTAGCGCTTATGA381250.2102158797158245No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCATT342740.18898200816734873No Hit
TAGCGTGTTACCTATACTCTACCGTCAGGGT334800.18460400401011948No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA334030.18417943685633276No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACCAT323650.17845605106892223No Hit
TTGATGATTCATAATAACTTTTCGAATCGC322270.1776951385075902No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGA320930.17695628138281852No Hit
CAGAGATGTATTTATTAGATAAAAAATCAATG317440.1750319445429281No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGA307590.1696007932899422No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTT304240.167753650478013No Hit
AAAGAGATGTATTTATTAGATAAAAAATCAATG303830.1675275822532694No Hit
AAGAGATGTATTTATTAGATAAAAAATCAAT301320.16614360360910754No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC300800.16585688293382297No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA290520.16018863573781333No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTG288830.15925679354313863No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCATT284780.15702368059140331No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGTT271210.14954137373830498No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG271040.1494476381329235No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT270520.14916091745763893No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA267160.14730826078656964No Hit
TGAAGAGATGTATTTATTAGATAAAAAATCA266000.14666865312631955No Hit
TCGGGTCTTGTAATTGGAATGAGTACAATGT264730.14596839301552847No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTT253350.13969362131410923No Hit
TAAGCGTGTTACCTATACTCTACCGTCAGGGT251160.13848608616243013No Hit
TGGACGTGGGTTAGTCGATCCTAAGAGATGGG247160.13628054250639524No Hit
GAGATGTATTTATTAGATAAAAAATCAATG244860.13501235490417518No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCAT240240.13246495198145491No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC238770.13165441468786207No Hit
TCAAGCGTGTTACCTATACTCTACCGTCAGGGT236910.13062883688780585No Hit
TAAGAGATGTATTTATTAGATAAAAAATCAAT235060.12960877294688974No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCC225240.1241941632713241No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATC222990.12295354496480448No Hit
TGGCCATCTTAGTATAGTGGTTAGTACACATCG221900.12235253431853498No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG218660.12056604395714672No Hit
TTGGTTCTATTTTGTTGGTTTCTAGGACCA215680.11892291393340074No Hit
AGGTTGGGGCCTCGCAAGAGGTGCACAATCG204490.11275290555564316No Hit
TAGATGTATTTATTAGATAAAAAATCAATG201770.11125313586953944No Hit
TTGACGTGGGTTAGTCGATCCTAAGAGATGGGG199510.11000700370387975No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCC199390.10994083739419869No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCT199070.10976439390171591No Hit
TTAGAGATGTATTTATTAGATAAAAAATCAATG197780.10905310607264465No Hit
TGGGCGTGTGGTCTAGTGGTATGATTCTCGCT197690.10900348134038387No Hit
TAATGACCCACTCGGCACCTTACGAGAAATC196890.1085623726091769No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA195450.10776837689300434No Hit
GGAAGAGATGTATTTATTAGATAAAAAATCA194470.10722801869727579No Hit
TTTGACCTCAAATCAGGTAGGAGTACCCGCTGA193780.10684756241660977No Hit
ACGTAGAGTTAAGGTGCCGGAATACACGCT193440.10666009120584681No Hit
CCATACGCGAAACTCAGGTGCTGCAATCT189020.10422296546592827No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTT188150.10374325972074068No Hit
TTTGTGCCCTCTGCTCCTTGTGGGTAGGGGA182800.10079334508079402No Hit
AAAAAGCTCGTAGTTGAACTTTGGGCCCGG181580.10012065426570338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)