FastQCFastQC Report
Fri 30 Jun 2023
SRR1944997.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944997.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21594355
Total Bases675 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGATGTATTTATTAGATAAAAAATCAATG11794885.462020051073533No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG7189443.3293145361368746No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT4525482.0956773193735123No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT3430351.5885401532020755No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC2818881.3053781879569915No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC2784221.289327696983772No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCT2773011.2841365254947417No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT2497691.1566402423225886No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCA2337891.0826394212746804No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA2100490.972703282871843No Hit
AGAGATGTATTTATTAGATAAAAAATCAAT1897230.8785768317692285No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT1856800.8598543461937159No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCAT1803110.8349913669567811No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCA1591100.7368129309720063No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGC1392480.644835189566903No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATC1379440.638796574382518No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATG1348110.6242881530844518No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG1268320.5873386817990165No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1265720.5861346634340318No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT1254150.5807767817098497No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG1178240.5456240762921606No Hit
TAGAGATGTATTTATTAGATAAAAAATCAAT1174450.543868987983202No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCA1140150.5279852072451342No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT1121720.5194505693733386No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGT1099000.5089293011993181No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG1093130.5062109981983718No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGT1003810.4648483365212807No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT952210.440953202816199No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTT869370.40259132537183906No Hit
TAAGAGATGTATTTATTAGATAAAAAATCAATG817250.3784553879937604No Hit
TTTGATGATTCATAATAACTTTTCGAATCGC717530.33227665285673036No Hit
AGATGTATTTATTAGATAAAAAATCAATG716280.3316977978735646No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACCA675070.31261410678855656No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA667490.3091039301706395No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAA637310.2951280554570859No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATC628510.2910529163755991No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGT624530.28920984210919937No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC610610.2827637130166657No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG597690.27678066791066464No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGT588930.2727240521886391No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTG550100.2547425009915786No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAAT545700.2527049314508352No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT542540.2512415860533922No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT525350.24328117232489693No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGTT511880.23704343102630296No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT499800.23144937646898925No Hit
TACCATACGCGAAACTCAGGTGCTGCAATCT487010.2255265322812374No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAA469600.21746424007570495No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTT457150.21169884444337422No Hit
TTGATGATTCATAATAACTTTTCGAATCGC456270.21129133053522553No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCT429040.1986815535819431No Hit
CAGAGATGTATTTATTAGATAAAAAATCAATG422030.1954353348363496No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA416500.19287448039082436No Hit
TCGGGTCTTGTAATTGGAATGAGTACAATGT412130.19085080336967694No Hit
AAGAGATGTATTTATTAGATAAAAAATCAAT407050.18849833671809138No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCT402420.18635425786044546No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG400660.1855392300441481No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTT394780.18281629620333648No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG394270.18258012337020485No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACC391250.18118160973087644No Hit
AAAGAGATGTATTTATTAGATAAAAAATCAATG384400.17800948442312817No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTG384190.17791223678595633No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCATT384140.1778890825866297No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCC377120.1746382330011709No Hit
ATGTACACGGAGTCGACCCGCAACGCGA360670.16702050142270977No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACCAT356070.16489031508465987No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCC354920.16435776850014738No Hit
GAGATGTATTTATTAGATAAAAAATCAATG351070.16257489515199688No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTT346690.16054658729098414No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATC342970.1588239148610829No Hit
TAATGACCCACTCGGCACCTTACGAGAAATC337920.15648534072909331No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT337040.15607782682094465No Hit
TTTGACCTCAAATCAGGTAGGAGTACCCGCTGA336710.1559250091053889No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGA336020.1556054811546814No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCAT332810.1541189815579118No Hit
TAGCGTGTTACCTATACTCTACCGTCAGGGT332020.15375314520855102No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGC326320.15111356648531526No Hit
TGAAGAGATGTATTTATTAGATAAAAAATCA322470.14933069313716477No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCATT320060.14821466072962125No Hit
TTTGCTCCACACAATATAGATGGATACGAAT317350.14695970312611792No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA313480.14516756809823678No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA309180.14317630695614664No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG307240.1422779240222734No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGTT299920.13888814924085485No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGC294430.1363458181547909No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTT292950.13566045385472267No Hit
TCAAGCGTGTTACCTATACTCTACCGTCAGGGT290220.1343962345714887No Hit
TGGACGTGGGTTAGTCGATCCTAAGAGATGGG288490.13359509927478733No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA282950.13102961398939675No Hit
TAAGCGTGTTACCTATACTCTACCGTCAGGGT280770.1300200908987557No Hit
GGAAGAGATGTATTTATTAGATAAAAAATCA275990.12780654944312994No Hit
TAAGAGATGTATTTATTAGATAAAAAATCAAT269190.12465757833470831No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCA267340.12380087295962301No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTC264610.12253665367638904No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGT256860.11894775278076145No Hit
TTTGTGCCCTCTGCTCCTTGTGGGTAGGGGA256430.11874862666655245No Hit
ATCAGCTTGCGTTGATTACGTCCCTGCCCTT254660.11792896801038975No Hit
TCGGGTCTTGTAATTGGAATGAGTACAATG250750.11611830962304731No Hit
AAAAAGCTCGTAGTTGAACTTTGGGCCCGG243480.1127516890409554No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCT242670.11237659101186398No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG240760.11149210059758673No Hit
TCTACATGGTATAACTGTGGTAATTCTAGAGCT237090.10979258236701213No Hit
AGAGATGTATTTATTAGATAAAAAATCAA234010.10836628368849174No Hit
TTAGAGATGTATTTATTAGATAAAAAATCAATG232280.10756514839179035No Hit
GCCTTGTTGGCGCAATCGGTAGCGCTTATGA231790.10733823723838938No Hit
TTTGCTCCACACAATATAGATGGATACGAATA229170.106124957193674No Hit
TCGGGTCTTGTAATTGGAATGAGTACAATGTA226520.10489778462936263No Hit
TAGTGACAATAAATAACGATACAGGGCCCA226330.10480979867192143No Hit
CCATACGCGAAACTCAGGTGCTGCAATCT225100.10424020536848634No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA224450.10393920077724016No Hit
TTATCTGGTTGATCCTGCCAGTAGTCATATGCT224010.10373544382316582No Hit
TAATGACCCACTCGGCACCTTACGAGAAATCA223990.10372618214343518No Hit
ATCAGCTTGCGTTGATTACGTCCCTGCCCT223810.10364282702585931No Hit
TGAGATGTATTTATTAGATAAAAAATCAATG222750.10315195800013476No Hit
TTAAATCAGTTATCGTTTATTTGATAGTTCC221030.10235545354329871No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG217610.10077170630935725No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCAGT216390.10020674384578748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)