FastQCFastQC Report
Fri 30 Jun 2023
SRR1944998.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944998.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23129075
Total Bases721.2 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGATGTATTTATTAGATAAAAAATCAATG21075199.111989995276508No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG12066025.216819090257609No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT6896462.9817275442273417No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCT6164572.6652903326224675No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC4300481.8593393812765966No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG3907251.6893239353497709No Hit
AGAGATGTATTTATTAGATAAAAAATCAAT3741121.61749659249235No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCA3629981.5694445195062925No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT3133291.3546974965492569No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT3031731.3107873963831238No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCAT2805531.2129884139335447No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT2520231.0896371774487306No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC2398241.0368940392125496No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCA2239260.9681580435015235No Hit
TAGAGATGTATTTATTAGATAAAAAATCAAT2186090.9451696619946971No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1982420.8571116657280933No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATC1907990.8249313904684905No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG1902980.8227652856847928No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTT1732660.7491263701639603No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1705390.7373360153832352No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATG1633680.706331749107995No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG1496900.6471940620193415No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCA1421520.6146030483277001No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGT1406090.607931791478907No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAA1303070.5633904511961676No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAA1238910.5356504745650226No Hit
TTGATGATTCATAATAACTTTTCGAATCGC1226440.5302589921992125No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA1183680.5117714391950391No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATC1180690.5104786940247287No Hit
TTTGATGATTCATAATAACTTTTCGAATCGC1109560.4797251943711541No Hit
AGATGTATTTATTAGATAAAAAATCAATG1019710.44087798582520055No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACC927480.4010017694179296No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGC926880.400742355671379No Hit
ATGTACACGGAGTCGACCCGCAACGCGA920190.39784989239733975No Hit
TAAGAGATGTATTTATTAGATAAAAAATCAATG908020.3925881169048049No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTT904390.39101866373817373No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT815860.3527421654346315No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAAT771260.3334590769410363No Hit
GAGATGTATTTATTAGATAAAAAATCAATG764960.33073523260225496No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGT763030.32990078505085046No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGT756980.32728502977313184No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT688290.2975864793555298No Hit
CAGAGATGTATTTATTAGATAAAAAATCAATG670230.2897781255843565No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG662200.28630630494302084No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT624690.27008862222116536No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATC608690.26317092231314915No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTT605840.26193870701703376No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTG596620.25795238244503943No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACCA580160.2508357986646677No Hit
TACCATACGCGAAACTCAGGTGCTGCAATCT569950.2464214414108649No Hit
AGAGATGTATTTATTAGATAAAAAATCAA565380.2444455733746378No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT543020.23477808775318512No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT541150.23396958157643571No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA503700.21777784022923527No Hit
AAGAGATGTATTTATTAGATAAAAAATCAAT502590.21729792479811666No Hit
TGAAGAGATGTATTTATTAGATAAAAAATCA489550.21165999937308344No Hit
TGAGATGTATTTATTAGATAAAAAATCAATG487960.21097255294472433No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG487720.2108687874461041No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTT474560.20517897927176076No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGA465800.20139153857212186No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTG446960.1932459469304328No Hit
GGAAGAGATGTATTTATTAGATAAAAAATCA433710.18751722669410686No Hit
TTTGCTCCACACAATATAGATGGATACGAAT428610.18531220984842672No Hit
AAAGAGATGTATTTATTAGATAAAAAATCAATG426810.18453396860877488No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGTT422620.18272239594536313No Hit
TGATGTATTTATTAGATAAAAAATCAATGT402030.1738201808762348No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAA363560.15718743615989833No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG356440.1541090597008311No Hit
TTAGAGATGTATTTATTAGATAAAAAATCAATG355780.1538237045796254No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAAT353080.1526563427201477No Hit
TCGGGTCTTGTAATTGGAATGAGTACAATGT348040.15047726724912258No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGC342940.1482722504034424No Hit
TTTTGGAAGAGATGTATTTATTAGATAAAAA335790.1451809032570477No Hit
TAGAGATGTATTTATTAGATAAAAAATCAA330850.14304506341044768No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC327760.14170908261571202No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG327310.14151452230579908No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC327100.14142372749450638No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGT322300.13934841752210153No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG315090.13623112900105172No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCT311140.1345233218362602No Hit
CCATACGCGAAACTCAGGTGCTGCAATCT310780.13436767358832982No Hit
GCCTTGTTGGCGCAATCGGTAGCGCTTATGA295030.12755806274137638No Hit
TAAGAGATGTATTTATTAGATAAAAAATCAAT294800.12745862080519865No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTC294390.12728135474505572No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA292290.1263734066321286No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTG286470.12385709329058772No Hit
AAGAGATGTATTTATTAGATAAAAAATCAA283640.12263352511935734No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGC279880.12100786564097354No Hit
AAGAGATGTATTTATTAGATAAAAAATCA279530.12085654095548568No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT260590.1126677136893715No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGT256910.11107664271052776No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACC256130.11073940484001196No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA253180.10946395391947149No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGTT251180.10859924143096947No Hit
TTTGCTCCACACAATATAGATGGATACGAATA250890.10847385812013668No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACCAT249850.10802420762611561No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA248160.1072935255733314No Hit
GAGAGATGTATTTATTAGATAAAAAATCAATG247480.1069995233272407No Hit
TCGGGTCTTGTAATTGGAATGAGTACAATG246910.10675308026801764No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCT246620.10662769695718485No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTG245630.10619966427537633No Hit
TAATGACCCACTCGGCACCTTACGAGAAATC243720.10537386384885691No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA242250.10473830016980792No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGA234790.10151292258769537No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC233690.10103733071901926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)