FastQCFastQC Report
Fri 30 Jun 2023
SRR1944999.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1944999.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28230699
Total Bases880.7 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGATGTATTTATTAGATAAAAAATCAATG14895255.276259719959467No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC7869392.7875292779679315No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG6609372.341199557262114No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCT4872221.7258587893980235No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA4407711.5613180530882356No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT3253601.1525042295268708No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT3137481.1113717021317822No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCA3092031.0952722070395777No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT3035601.075283328974603No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT2944191.0429036843898196No Hit
AGAGATGTATTTATTAGATAAAAAATCAAT2758330.9770675533042947No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCA2697510.9555236304988409No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT2675400.9476917309061316No Hit
TCCGATATAGTGTAACGGCTATCACATCACG2554420.9048376733427679No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG2501070.8859398061663297No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC2460060.8714130670303275No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCAT2448400.8672828115237246No Hit
ATGTACACGGAGTCGACCCGCAACGCGA2391890.8472655955135932No Hit
AGATGTATTTATTAGATAAAAAATCAATG2224660.7880286634064568No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGC2140660.7582738209918217No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGT2116150.7495917830444085No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATG2086320.7390252717440684No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATC1923550.6813681800794235No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG1844250.6532781919427499No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGT1605070.5685548204102208No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCA1468710.520252792890463No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTT1388820.49195381240825814No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG1263710.44763680842617465No Hit
TAGAGATGTATTTATTAGATAAAAAATCAAT1197260.4240986027303114No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA1133660.40156993633065907No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACCA1100490.38982031582002274No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT1083760.3838941430391079No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC1031700.36545322522832324No Hit
TAAGAGATGTATTTATTAGATAAAAAATCAATG975160.34542538248875804No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA900430.31895419946916653No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCT893950.3166588259114661No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAA850190.301157969910699No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAA820230.2905454094494791No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACC772730.2737197545126318No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTT771110.2731459111232067No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATC728640.2581020044880929No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTT725260.2569047263052183No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATCA706250.25017092208733477No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG699030.247613422536934No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGT688360.2438338491016464No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGTT672140.2380883307211061No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAAT669840.23727361479784825No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG655410.23216215794019127No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT652710.23120575229114942No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT638830.22628911880644542No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGC590340.20911278179828277No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGA589630.20886128253501623No Hit
AAGAGATGTATTTATTAGATAAAAAATCAAT583790.20679261253857018No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT583230.20659424692247258No Hit
AGAGATGTATTTATTAGATAAAAAATCAA575220.20375691016364844No Hit
TTTGATGATTCATAATAACTTTTCGAATCGC574290.2034274815512007No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT561750.198985508647873No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCC558310.19776697700613083No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCT553690.19613046067332587No Hit
GAGATGTATTTATTAGATAAAAAATCAATG551210.1952519843734652No Hit
TAATGACCCACTCGGCACCTTACGAGAAATC547460.19392364319424044No Hit
AAAGAGATGTATTTATTAGATAAAAAATCAATG534620.18937540299657477No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTG523140.1853089078665746No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATC509630.18052333737822077No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC496330.17581215399590355No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACCAT467590.16563174719832477No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA466390.16520667802097283No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTG464850.16466117257670454No Hit
TACCATACGCGAAACTCAGGTGCTGCAATCT461250.16338596504464872No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA446390.158122191731774No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTT438070.15517504543546726No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGTT430470.1524829406455717No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTG426580.1511050080623225No Hit
TTGATGATTCATAATAACTTTTCGAATCGC425960.15088538898735734No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG420270.14886985263808028No Hit
CCATACGCGAAACTCAGGTGCTGCAATCT418760.14833497392324574No Hit
TAGATGTATTTATTAGATAAAAAATCAATG413010.14629818411510107No Hit
TCGGGTCTTGTAATTGGAATGAGTACAATGT410070.14525676463058884No Hit
TTTGCTCCACACAATATAGATGGATACGAAT406700.14406302869085885No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA403100.14278782115880306No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT396700.1405207855462594No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACC381370.1350905268055885No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGT370730.13132158009973469No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG361030.12788560424947323No Hit
CAGAGATGTATTTATTAGATAAAAAATCAATG357700.12670603728232163No Hit
TGAAGAGATGTATTTATTAGATAAAAAATCA356150.12615698959490873No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTG353660.12527497105190347No Hit
ATCCGATATAGTGTAACGGCTATCACATCACGC346890.12287687244300965No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAT346610.12277768963496086No Hit
AAGAGATGTATTTATTAGATAAAAAATCA344910.12217550830037896No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTC342580.12135016564768729No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTT342290.12124744059649391No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGC334620.11853054010458615No Hit
AAGAGATGTATTTATTAGATAAAAAATCAA332410.11774770436962967No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCT331720.11750328959265231No Hit
AAAAAGCTCGTAGTTGAACTTTGGGCCCGG321300.1138122722359797No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCATT313130.11091825958684197No Hit
GGAAGAGATGTATTTATTAGATAAAAAATCA312800.11080136556307017No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACC312290.11062071116269563No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCAT310950.11014605058131928No Hit
GGTCCTATAGTGTAGTGGTTATCACTTTCGGT310380.10994414272207712No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG300070.10629209003999512No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGT296830.10514440326114491No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCATT295540.10468745389549158No Hit
TCTACATGGTATAACTGTGGTAATTCTAGAGCT291500.1032563876650734No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGA291290.10318200055903681No Hit
TGAGATGTATTTATTAGATAAAAAATCAATG288010.1020201448076082No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA285880.10126564701780853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)