FastQCFastQC Report
Fri 30 Jun 2023
SRR1945000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1945000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18134196
Total Bases561 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGATGTATTTATTAGATAAAAAATCAATG10024275.527827095284511No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC4201452.316865881454022No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT4063332.240700387268341No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG4055972.236641756822304No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCT3248611.79142764311139No Hit
AGATGTATTTATTAGATAAAAAATCAATG2903341.6010304509778102No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT2279801.2571828384340833No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA2235581.2327979690966173No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT2019021.113377179776815No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT2001101.1034952969516818No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCAT1914661.055828446984912No Hit
AGAGATGTATTTATTAGATAAAAAATCAAT1881201.0373771188973584No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT1854361.0225763524338218No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG1763860.9726706383894825No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1664760.9180225029000458No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC1551060.8553232798410252No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG1485630.8192422757534991No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCA1320810.7283532173138527No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCA1258080.6937611129823457No Hit
ATGTACACGGAGTCGACCCGCAACGCGA1196970.6600623485044499No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGC1126110.6209870015742633No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCA1073530.5919920574366794No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATG996230.5493654088662105No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGT981610.5413032924095449No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATC880000.48527103159136475No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA866700.4779368216820862No Hit
AGAGATGTATTTATTAGATAAAAAATCAA849860.4686504987593605No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT834450.46015274126297084No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG829860.45762161167773857No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACCA821250.45287367578910037No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAA794870.43832657372844097No Hit
TAGAGATGTATTTATTAGATAAAAAATCAAT771560.42547240583481066No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTT751220.4142560276728011No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGT709800.3914152025267621No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTT700770.3864356600094098No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC622340.34318587931882943No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACC558860.3081801917217615No Hit
TAGATGTATTTATTAGATAAAAAATCAATG533940.2944381984180605No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC527680.2909861567615129No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTT521210.28741831179060817No Hit
AAGAGATGTATTTATTAGATAAAAAATCA483360.2665461429886387No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA482620.26613807416661867No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCT477020.26304998578376454No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAA474370.26158865824544963No Hit
TTTGATGATTCATAATAACTTTTCGAATCGC444400.24506187095363918No Hit
GAGATGTATTTATTAGATAAAAAATCAATG435270.24002718400087877No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT425590.23468920265337376No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG412730.22759762825989086No Hit
TGATGTATTTATTAGATAAAAAATCAATGT408760.22540839417418893No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG404400.22300409679039535No Hit
TAAGAGATGTATTTATTAGATAAAAAATCAATG402630.2220280402836718No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT381230.2102271311063363No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATC373160.2057769751689019No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACC372670.20550676743540217No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT369000.20348296665592455No Hit
TGGTTCTATTTTGTTGGTTTCTAGGACCA366760.2022477313027829No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTT357130.19693732217298193No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAAT342990.1891398990062752No Hit
TAGAGATGTATTTATTAGATAAAAAATCAA342740.18900203791775494No Hit
AAGAGATGTATTTATTAGATAAAAAATCAAT331080.18257219674916936No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGC329940.18194355018551692No Hit
TTGATGATTCATAATAACTTTTCGAATCGC326420.18000246605915146No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACCAT324800.17910912620554006No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATC310160.17103598086179284No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAT300690.16581380282864486No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTG295780.16310621105010667No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGT293620.1619150912452915No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAT289780.15979754492562007No Hit
TTGGTTCTATTTTGTTGGTTTCTAGGACCA286170.15780683080738733No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG282170.15560105339106295No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGT277970.15328498710392233No Hit
TTTGCTCCACACAATATAGATGGATACGAAT271900.1499377198746501No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGA267910.14773745690186652No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACC265970.14666765485494918No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC262680.14485340293002238No Hit
CCATACGCGAAACTCAGGTGCTGCAATCT259300.14298952101322826No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTTG255100.14067345472608767No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG254470.14032604478301658No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCT246970.13619021212740837No Hit
TACCATACGCGAAACTCAGGTGCTGCAATCT246230.13578214330538835No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA245360.1353023867173378No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCA245100.1351590111852767No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG242750.13386311695318612No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT242740.13385760250964532No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA242390.13366459698571692No Hit
TAATGACCCACTCGGCACCTTACGAGAAATC239220.13191651838327986No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGTT235020.12960045209613924No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCC232200.12804537901763058No Hit
TAAGAGATGTATTTATTAGATAAAAAATCA230630.12717961138172323No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT229160.12636898818122402No Hit
TGAAGAGATGTATTTATTAGATAAAAAATCA227960.1257072549563267No Hit
CAGAGATGTATTTATTAGATAAAAAATCAATG225590.12440033183715452No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTT221720.12226624218686066No Hit
TCGGGTCTTGTAATTGGAATGAGTACAATGT220950.12184163003421823No Hit
TGAGATGTATTTATTAGATAAAAAATCAATG220720.12171479783277958No Hit
AAGAGATGTATTTATTAGATAAAAAATCAA210840.11626652761445834No Hit
GCCTTGTTGGCGCAATCGGTAGCGCTTATGA207870.11462873788283749No Hit
AAAGAGATGTATTTATTAGATAAAAAATCAATG206000.11359753694070583No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTG205950.11356996472300178No Hit
AGGTTGGGGCCTCGCAAGAGGTGCACAATCG204050.11252222045024769No Hit
TGATGTATTTATTAGATAAAAAATCAATG203500.11221892605550308No Hit
ATGTATTTATTAGATAAAAAATCAATG200570.11060319409804548No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCTTG200270.11043776079182116No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACC197540.10893231770517976No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTT195840.10799486230324189No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCATT190340.10496191835579587No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGT189070.10426158402611287No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA187280.10327449863230771No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGT185900.10251350542367581No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCAT185130.10208889327103336No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTC183640.10126724118345252No Hit
TATCTGGTTGATCCTGCCAGTAGTCATATGCT182450.10061102240209602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)