FastQCFastQC Report
Fri 30 Jun 2023
SRR1945001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1945001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32837073
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length25-48
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGATGTATTTATTAGATAAAAAATCAATG14373794.3773054924840595No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATG6723122.0474175636787115No Hit
TCCGATATAGTGTAACGGCTATCACATCACGC5496011.6737210408491645No Hit
AGATGTATTTATTAGATAAAAAATCAATG5295181.6125615093647354No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA4712161.4350121888147582No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGT3465491.0553589840361228No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT2893750.8812448052236568No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATCA2800340.8527982990444976No Hit
AGAGATGTATTTATTAGATAAAAAATCAAT2668790.8127368721323No Hit
ATGTACACGGAGTCGACCCGCAACGCGA2527830.7698097817670899No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATCA2190420.6670570181453139No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCG2120330.6457122411610804No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT2035880.6199943582060435No Hit
AAGAGATGTATTTATTAGATAAAAAATCAATG2029790.6181397471083979No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT1937560.5900525908627727No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACCA1889190.5753222889262999No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACCT1872560.5702578911342068No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTC1870770.5697127755570662No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC1853460.5644412947524282No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAATC1613580.49138971673876053No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTT1584660.4825825980287585No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCT1522890.4637715426097813No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGC1521810.46344264606044516No Hit
AACGGGGCCTTTCCTTCTGGCTAACCT1505520.4584817897746245No Hit
TCCGATATAGTGTAACGGCTATCACATCACG1424740.4338815460196468No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGT1401600.42683463291627727No Hit
TAGAGATGTATTTATTAGATAAAAAATCAAT1219590.37140642833787285No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCA1140700.3473817535442334No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCG1120310.34117230850630326No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAA1116970.3401551654740969No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGT1094390.3332787913222351No Hit
TAGATGTATTTATTAGATAAAAAATCAATG1065870.32459348614902434No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA1036570.31567064457907074No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGC1030070.31369117460621415No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACC1024620.31203146516743435No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGG1023390.3116568885418015No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCT964330.29367111983458455No Hit
TAAGAGATGTATTTATTAGATAAAAAATCAATG955470.29097294999466No Hit
AGAGATGTATTTATTAGATAAAAAATCAA830050.25277831553378705No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTG787800.2399117607102192No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAA763880.23262731121010696No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCT750900.22867446194123334No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTTG733360.22333293835294027No Hit
TTGGAAGAGATGTATTTATTAGATAAAAAATC732770.2231532633861733No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCC722350.21998002075276318No Hit
TATACGCGAAACTCAGGTGCTGCAATCT718950.21894460569003824No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCAT717410.2184756235733922No Hit
AAGAGATGTATTTATTAGATAAAAAATCA714860.21769906227634847No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGC704440.21452581964293832No Hit
TGATGTATTTATTAGATAAAAAATCAATG695230.21172106295832155No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGT677090.20619681906484175No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT674600.2054385297983167No Hit
TCGGGTCTTGTAATTGGAATGAGTACAATGT669330.20383363645109295No Hit
TTGGTTCTATTTTGTTGGTTTCTAGGACCA668080.20345296914862054No Hit
TTTGGAAGAGATGTATTTATTAGATAAAAAAT660600.20117505601062552No Hit
AACTATGCCGACTAGGGATCGGGTGGTGT642100.19554117993403372No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACCAT640960.19519401135417885No Hit
TGGTTCTATTTTGTTGGTTTCTAGGACCA638690.19450271953288895No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTT637710.19420427636775056No Hit
TAGAGATGTATTTATTAGATAAAAAATCAATGT635200.19343989642438594No Hit
ATTTTCCACGTTCTAGCATTCAAGGTCCCA630900.1921304009038808No Hit
TCCGATATAGTGTAACGGCTATCACATC604560.18410897950618194No Hit
AAGAGATGTATTTATTAGATAAAAAATCAAT594080.18091746484225313No Hit
TAATGACCCACTCGGCACCTTACGAGAAATC589040.17938261427868435No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTG585750.17838069793857692No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGG560840.1707947599349065No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGTT558900.1702039642814693No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGC555900.1692903627555355No Hit
GAGATGTATTTATTAGATAAAAAATCAATG550950.1677829202377447No Hit
AGAGATGTATTTATTAGATAAAAAATC515550.1570024222317257No Hit
AAAAAGCTCGTAGTTGAACTTTGGGCCCGG505250.15386572365935297No Hit
AAAGAGATGTATTTATTAGATAAAAAATCAATG505210.15385354230567383No Hit
TGGAAGAGATGTATTTATTAGATAAAAAATC487950.1485972881931346No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGA484380.14751010237727338No Hit
TTTGATGATTCATAATAACTTTTCGAATCGC482960.14707766432166472No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCT474990.14465052960110056No Hit
ATGCAAGAGTTCGAATCTCTTAGCAACCACC472350.1438465602582788No Hit
TTGAGATTAAGCCTTTGTTGTCTGATTTGT471990.14373692807516675No Hit
TTTGTGCCCTCTGCTCCTTGTGGGTAGGGGA471110.14346893829422616No Hit
TTTCCAACGGGGCCTTTCCTTCTGGCTAACC467930.14250052067673633No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACT460340.1401891088161238No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCA449980.13703413821323235No Hit
GGTCCTATAGTGTAGTGGTTATCACTTTCGGT448800.13667478827969837No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT447350.1362332142088304No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCA432150.1316042998107657No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTC430050.13096477874261206No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGT429780.130882554605278No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGA429490.1307942397911044No Hit
AGGGGGCATGCCTGTTTGAGCGTCATT428690.13055061271752205No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTT428470.1304836152722869No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCC423280.12890308463242142No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAT416800.12692970533640438No Hit
TTGCAAGAGTTCGAATCTCTTAGCAACCACC411810.12541008146493446No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGG411560.12533394800443998No Hit
TTTGGTTCTATTTTGTTGGTTTCTAGGACC406330.12374123601089537No Hit
ACGTAGAGTTAAGGTGCCGGAATACACGCT406010.12364378518146242No Hit
TTTTGGTTCTATTTTGTTGGTTTCTAGGACCA404690.12324180051005156No Hit
TAAACTATGCCGACTAGGGATCGGGTGGTGT402810.12266927688713303No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAA397600.12108265557042798No Hit
TTCCGATATAGTGTAACGGCTATCACATCACG396600.12077812172845002No Hit
TAAGAGATGTATTTATTAGATAAAAAATCA379000.11541832610963833No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT376440.11463871947417481No Hit
AAAACTATGCCGACTAGGGATCGGGTGGTGTT376040.11451690593738365No Hit
AAGAGATGTATTTATTAGATAAAAAATC373270.11367334719510475No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGG370400.11279933506862806No Hit
ATGCAGGCCACCATCGAAAGGGAATCCGGTT370200.11273842830023248No Hit
TAGCGTGTTACCTATACTCTACCGTCAGGGT360630.10982403943250363No Hit
TAGAGATGTATTTATTAGATAAAAAATCAA359720.10954691363630369No Hit
CAGAGATGTATTTATTAGATAAAAAATCAATG358000.10902311542810164No Hit
AGGTTGGGGCCTCGCAAGAGGTGCACAATCG357920.10899875272074341No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGC346490.10551793090693559No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGC342620.10433938493848098No Hit
TCGGGTCTTGTAATTGGAATGAGTACAATG340640.10373640793136464No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGC334980.10201274638576953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)