FastQCFastQC Report
Fri 30 Jun 2023
SRR1945002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1945002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44431539
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length25-49
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGA695565315.654764963239288No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGT15433503.4735461222713893No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAA10129412.279779235195972No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGA9413552.1186639517483288No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGA6380781.4360925017699702No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGT5240881.179540506125615No Hit
ACTTTGGTCGTGACTTCTAATTTGCTGGT4903201.1035404377957738No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGA3124910.7033089715843514No Hit
ATAGCCCAACGCAAGGCGGGGTGCAATTATTAGA2512360.5654451897333559No Hit
ATATCCCAACGCAAGGCGGGGTGCAATTATTAGA2365360.5323605828733504No Hit
TCGCCTTTTTTCGAAGCATTAAGCGGAGCCA2258490.5083078486207736No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTAGTT2188420.49253751935083767No Hit
TCAATGAGTTGAATACAAATGATTCTTCGA2066700.465142564609342No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATT2034860.4579764837765354No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTG2003820.45099045522596015No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAA1887620.4248378612318605No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTT1604890.36120513403778337No Hit
TCAATGAGTTGAATACAAATGATTCTTCG1458360.3282263078935888No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGT1441800.32449922565140044No Hit
TACTGTGGTCGTGACTTCTAATTTGCTGGT1407860.31686050757773665No Hit
TCGCCTTTTTTCGAAGCATTAAGCGGAGCC1293210.2910567648804603No Hit
GTGGGTTCGTGGCGGCTAGCGTTTAGTT1041420.23438755970167946No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGG989070.22260538848316733No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAG946250.21296809007673578No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGG863630.194373190629296No Hit
CTGGGGTCGTGACTTCTAATTTGCTGGT827900.18633160557413958No Hit
TCGGCCTTTTTTCGAAGCATTAAGCGGAGCC770250.1733565879858449No Hit
TTCAATGAGTTGAATACAAATGATTCTTCG756130.1701786652044621No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGA724770.16312061574099426No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATT704820.15863056195285066No Hit
TCTGTGGTCGTGACTTCTAATTTGCTGGT696710.15680528194173063No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGT686050.15440608528099828No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCT633380.1425518931495936No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAA624900.14064333895794157No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCATT569830.12824898997984294No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGA540760.12170634017426224No Hit
ATTCCCCAACGCAAGGCGGGGTGCAATTATTAGA539800.12149027743558467No Hit
ATGCCCCAACGCAAGGCGGGGTGCAATTATTAGA539710.12147002155383366No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGA534330.120259169955828No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTT526720.11854642262110254No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGA519760.11697996776569004No Hit
TTAACTGTGGTCGTGACTTCTAATTTGCTGGT499370.11239088522231923No Hit
TTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTG478930.10779054941130893No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGA455160.10244074597551077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)