FastQCFastQC Report
Fri 30 Jun 2023
SRR1945003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1945003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27016372
Total Bases812.4 Mbp
Sequences flagged as poor quality0
Sequence length25-47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTGGTCGTGACTTCTAATTTGCTGGT749743427.75144641923053No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGT14900165.515233503595524No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGT12147894.496491979011838No Hit
TCGCCTTTTTTCGAAGCATTAAGCGGAGCCA8359693.0943051864995046No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTT7596212.811706175795921No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGA5944532.200343554641608No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTT5886352.178808464733903No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGG5724012.1187189752939437No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGT5439672.013471683022428No Hit
TACTGTGGTCGTGACTTCTAATTTGCTGGT4620151.710129694690316No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCATT4513061.670490767598255No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAA4349171.6098275519747802No Hit
CTTTTTTCGAAGCATTAAGCGGAGCCATT4125321.52697038669737No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGT4112811.5223398611775112No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGG3490531.2920054550625821No Hit
TTAACTGTGGTCGTGACTTCTAATTTGCTGGT3428921.2692007646326458No Hit
CCTTTTTTCGAAGCATTAAGCGGAGCCATT2339570.8659823013985742No Hit
TCAACTGTGGTCGTGACTTCTAATTTGCTGGT2306790.8538489179820296No Hit
TCTTTTTTCGAAGCATTAAGCGGAGCCATT2089650.7734754318603548No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGA1876520.6945862308973241No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAA1450940.5370595282001596No Hit
CAACTGTGGTCGTGACTTCTAATTTGCTGGT1414660.523630634046644No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGG1357570.5024990031970245No Hit
CCCTTTTTTCGAAGCATTAAGCGGAGCCATT1289640.47735499052204344No Hit
TCGCCTTTTTTCGAAGCATTAAGCGGAGCCAT1228110.454579911766095No Hit
TCGCCTTTTTTCGAAGCATTAAGCGGAGCC1188900.4400664900527725No Hit
TCAACTGTGGTCGTGACTTCTAATTTGCTGG1061200.392798855449577No Hit
TCTGTGGTCGTGACTTCTAATTTGCTGGT1019360.377311949953902No Hit
ACGGTGGTCGTGACTTCTAATTTGCTGGT961270.3558101731794336No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGG912480.3377507535060592No Hit
TTGTGGTCGTGACTTCTAATTTGCTGGTT888680.3289412804946571No Hit
TGGCCTTTTTTCGAAGCATTAAGCGGAGCCA867530.3211126941841043No Hit
TCGGCCTTTTTTCGAAGCATTAAGCGGAGCCA866550.3207499511777525No Hit
TTAACTGTGGTCGTGACTTCTAATTTGCTGG831870.30791329050399513No Hit
TCGGCCTTTTTTCGAAGCATTAAGCGGAGCC806480.2985152854720834No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAA805020.2979748724218041No Hit
CTTTGGTCGTGACTTCTAATTTGCTGGT740750.2741855938317699No Hit
GTGGGTTCGTGGCGGCTAGCGTTTAGTT735180.2721238810303619No Hit
CTGTGGTCGTGACTTCTAATTTGCTGGTTT704920.26092326534443633No Hit
TCGGCCTTTTTTCGAAGCATTAAGCGGAGC691520.255963309951462No Hit
CCGCCTTTTTTCGAAGCATTAAGCGGAGCCA646280.23921790831130102No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCATT644240.23846281062460942No Hit
GCCTTTTTTCGAAGCATTAAGCGGAGCCA622640.2304676586478747No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTAGTT614560.22747687957509616No Hit
TTGTGGTCGTGACTTCTAATTTGCTGGT589720.2182824548018513No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTT562400.20817006813498126No Hit
TCGCCTTTTTTCGAAGCATTAAGCGGAGC540370.20001575341056158No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTTT538170.19920143237589413No Hit
TCTGTGGTCGTGACTTCTAATTTGCTGGTT498940.1846806077440746No Hit
TACTGTGGTCGTGACTTCTAATTTGCTGGTT491520.18193412498169628No Hit
TGGCCTTTTTTCGAAGCATTAAGCGGAGCC449140.1662473406866029No Hit
TTTGTGCTTGCTTGCGGACGCTTTCTGGTG442980.16396724178953415No Hit
TCTTCTAATTTGCTGGTTTGAGGTTGGGT408340.15114538695277072No Hit
TTTGTGCTTGCTTGCGGACGCTTTCTGGT384650.14237662999310197No Hit
ATGTACACGGAGTCGACCCGCAACGCGA382780.14168445711363464No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAA379220.14036673762117283No Hit
ATTGTGCTTGCTTGCGGACGCTTTCTGGT365890.13543269244293793No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGA365270.13520320196953164No Hit
CTTTGGTCGTGACTTCTAATTTGCTGGTT363800.13465908746000388No Hit
GCGCCTTTTTTCGAAGCATTAAGCGGAGCCA355390.13154616023202523No Hit
TACTGTGGTCGTGACTTCTAATTTGCTGG348610.12903657086155018No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGA338200.12518335178387385No Hit
CCCTTTTTTCGAAGCATTAAGCGGAGCCAT321300.11892788565392866No Hit
CAACTGTGGTCGTGACTTCTAATTTGCTGG319760.11835786092966147No Hit
TTGTGCTTGCTTGCGGACGCTTTCTGGTG312150.11554105044156188No Hit
CTGTGGTCGTGACTTCTAATTTGCTGG286150.10591725639549232No Hit
TTTTTTCGAAGCATTAAGCGGAGCCATT279620.10350020350622947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)